Compositions and methods for co-amplifying subsequences of a nucleic acid fragment sequence
The present invention is related to genomic nucleotide sequencing. In particular, the invention describes a single reaction method to co-amplify multiple subsequences of a nucleic acid fragment sequence (i.e., for example, at least two read pairs from a single library insert sequence). Nucleic acid fragment sequences may include, but are not limited to, localizing library insert sequences and/or unique read pair sequences in specific orientations on a single emulsion polymerase chain reaction bead. Methods may include, but are not limited to, annealing, melting, digesting, and/or reannealing high throughput sequencing primers to high throughput sequencing primer binding sites. The compositions and methods disclosed herein contemplate sequencing complex genomes, amplified genomic regions, as well as detecting chromosomal structural rearrangements that are compatible with massively parallel high throughput sequencing platforms as well as ion semiconductor matching sequencing platforms (i.e., for example, Ion Torrent platforms).
1. A method, comprising:
a) providing:
i) a solid substrate that can be attached to at least one nucleic acid sequence bearing an attachment feature;
ii) a plurality of random primers comprising random primers bearing an attachment feature and lacking a universal sequence and random primers that do not bear said attachment feature, wherein the ratio of random primers bearing the attachment feature to random primers not bearing the attachment feature is between 30:70 and 70:30; and
iii) a plurality of nucleic acid fragments, comprising a plurality of subsequences and lacking terminal universal sequences;
b) annealing said plurality of nucleic acid fragments to said plurality of random primers under conditions that generate a plurality of linear amplified nucleic acid fragments incorporating said attachment feature; and
c) combining the solid substrate under conditions that attach the solid substrate, through the attachment feature, with either
(1) the plurality of random primers of (a)(ii) prior to step (b), or
(2) the plurality of linear amplified nucleic acid fragments generated in step (b).
2. The method of claim 1 , wherein said attachment feature is biotin.
3. The method of claim 1 , wherein said solid substrate is selected from the group consisting of a bead, a microwell, and a surface.
4. The method of claim 1 , wherein said plurality of random primers are hexamers, heptamers, octamers or nonamers.
5. The method of claim 1 , wherein said plurality of nucleic acid fragments are derived from a biological sample selected from the group consisting of a single genome, a single nucleic acid library, and a single nucleic acid library insert sequence.
6. The method of claim 1 , wherein each of said plurality of nucleic acid fragments is circularized.
7. A method, comprising:
a) providing:
i) a solid substrate that can be attached to at least one nucleic acid sequence bearing an attachment feature;
ii) a plurality of random primers each bearing an attachment feature and lacking a universal sequence; and
iii) a plurality of nucleic acid fragments, comprising a plurality of subsequences and lacking terminal universal sequences;
b) annealing said plurality of nucleic acid fragments to said plurality of random primers under conditions that generate a plurality of linear amplified nucleic acid fragments incorporating said attachment feature; and
c) combining the solid substrate under conditions that attach the solid substrate, through the attachment feature, with the plurality of random primers of (a)(ii) prior to step (b).
8. A method, comprising:
a) providing:
i) a solid substrate that can be attached to at least one nucleic acid sequence bearing an attachment feature;
ii) a plurality of random primers each bearing an attachment feature and lacking a universal sequence; and
iii) a plurality of nucleic acid fragments, comprising a plurality of subsequences and lacking terminal universal sequences, wherein each of said plurality of nucleic acid fragments is ligated to at least one barcode, thereby generating a barcoded nucleic acid fragment;
b) annealing said plurality of nucleic acid fragments to said plurality of random primers under conditions that generate a plurality of linear amplified nucleic acid fragments incorporating said attachment feature; and
c) combining the solid substrate under conditions that attach the solid substrate, through the attachment feature, with either
(1) the plurality of random primers of (a)(ii) prior to step (b), or
(2) the plurality of linear amplified nucleic acid fragments generated in step (b).
9. The method of claim 8 , wherein said barcoded nucleic acid fragment is sequenced.
10. A method, comprising:
a) providing:
i) a solid substrate that can be attached to at least one nucleic acid sequence bearing an attachment feature;
ii) a plurality of random primers each bearing an attachment feature and lacking a universal sequence; and
iii) a plurality of nucleic acid fragments, comprising a plurality of subsequences and lacking terminal universal sequences, wherein said plurality of subsequences comprises a first subsequence having a first read pair sequence, and wherein said first read pair sequence comprises a first high throughput sequencing primer binding site;
b) annealing said plurality of nucleic acid fragments to said plurality of random primers under conditions that generate a plurality of linear amplified nucleic acid fragments incorporating said attachment feature; and
c) combining the solid substrate under conditions that attach the solid substrate, through the attachment feature, with either
(1) the plurality of random primers of (a)(ii) prior to step (b), or
(2) the plurality of linear amplified nucleic acid fragments generated in step (b).
11. The method of claim 10 , wherein said method further provides a first high throughput sequencing primer.
12. The method of claim 11 , wherein said method further comprises the step of annealing said first high throughput sequencing primer binding site to said first high throughput sequencing primer, under conditions such that said first read pair sequence is amplified.
13. The method of claim 10 , wherein said plurality of subsequences comprises a second subsequence having a second read pair sequence, and wherein said second read pair sequence comprises a second high throughput sequencing primer binding site.
14. The method of claim 13 , wherein said method further provides at least one primer selected from the group consisting of a first high throughput sequencing primer and a second high throughput sequencing primer.
15. The method of claim 14 , wherein said method further comprises the step of annealing said second high throughput sequencing primer binding site to said second high throughput sequencing primer, under conditions such that said second read pair sequence is amplified.
16. The method of claim 14 , wherein said first and second high throughput sequencing primers are compatible with ion semiconductor sequencing, pyrosequencing, polymerase-based sequence-by-synthesis, and ligation-based sequencing.
17. A method, comprising:
a) providing:
i) a solid substrate that can be attached to at least one nucleic acid sequence bearing an attachment feature;
ii) a plurality of random primers lacking a universal sequence, wherein the plurality of random primers comprises random primers bearing an attachment feature and random primers that do not bear an attachment feature, and wherein the ratio of random primers bearing the attachment feature to random primers not bearing the attachment feature is between 30:70 and 70:30; and
iii) a plurality of nucleic acid fragments, each comprising a plurality of subsequences and lacking terminal universal sequences;
b) annealing said plurality of nucleic acid fragments to said plurality of random primers under conditions that generate a plurality of amplified nucleic acid fragments incorporating said attachment feature; and
c) combining the solid substrate under conditions that attach the solid substrate, through the attachment feature, with either
(1) the plurality of random primers of (a)(ii) prior to step (b), or
(2) the plurality of amplified nucleic acid fragments after step (b).