Nanobiocomposite formulation for wound healing and a process for the preparation thereof
The present invention involves the isolation of plant based CNCs from the leaves of S. cumini . For the formation of NCs, a novel greener approach using S. cumini LE as reducing agent for in situ impregnation of AgNPs as fillers into CNCs as matrix is reported. The silver nitrate solution in three different concentrations of 1 mM, 5 mM and 10 mM was used to form NCs where AgNPs have been incorporated into CNCs matrix. The CNCs and NCs were characterized using SEM, TEM, XRD, Zeta potential, FT-IR, and UV-Vis spectroscopy. NCs developed in the form of film and ointment showed strong antimicrobial activity against both gram negative and gram positive bacteria. NCs wound dressing is capable of regulating wound exudates and providing moisture to wound responsible for faster healing of acute wounds. The observations from histopathological and biochemical assays confirmed that NCs enhance healing because of lesser inflammation, rapid angiogenesis, early collagen formation and enhanced rate of reepithelization.
1. A nanobiocomposite formulation (NCs) in ointment form comprising silver nanoparticles (AgNPs) and cellulose nanocrystals (CNCs) wherein the ratio of AgNPs and CNCs is in the range of 0.067%-0.4% w/w AgNPs: 7-8% w/w CNCs, and wherein the cellulose is derived from Syzygium cumini leaves.
2. A process for the preparation of the nanobiocomposite formulation in ointment form as claimed in claim 1 , the method comprising:
a) treating washed and dried Syzygium cumini leaves with bleaching agent at pH in the range of 3.0-4.0 for a time period ranging between 2-3 h at a temperature ranging between 70−100° C. to obtain a bleached fibrous material;
b) filtering and washing the bleached fibrous material as obtained from (a) followed by treating with acidified bleaching agent solution at a temperature ranging between 22-30° C. for a time period ranging 16-18 h to obtain acidified fibrous material;
c) washing the acidified fibrous material as obtained from (b) followed by keeping it in 2-18% base for 2 to 5 h at 70 to 80° C. under continuous stirring to obtain fibrous material;
d) treating the fibrous material from (c) with 0.6 to 3% sodium chlorite solution for a time period ranging from 0.5 to 3 h at a temperature ranging from 80-100° C. followed by filtrating and washing to obtain a chemically treated fiber;
e) treating the chemically treated fiber as obtained from (d) with 64-67% (v/v) acid in the ratio ranging from 1:5-1:9 for a time period ranging from 30-120 min at a temperature ranging from 40 to 80° C. under continuous stirring to obtain acidified chemically treated fiber;
f) centrifuging and dialyzing the acidified chemically treated fiber as obtained from (e) against water to remove the acid content until neutral to obtain the CNCs;
g) treating the CNCs as obtained from (f) mechanically for a time period ranging from 4-30 min to individualize the fibers and freeze drying to obtain white solid CNCs;
h) dipping Syzygium cumini leaves in water for a period of time in the range of 20-28 h at a temperature in the range 20-30° C. followed by filtration and storing at a temperature 4-10° C. to obtain leaf extract as a biological reducing agent;
i) freeze drying of the CNCs as obtained from (g) and adding each of 1 mM, 5 mM and 10 mM silver nitrate solution and sonicating for 2 to 5 min to obtain a mixture;
j) adding 10-20% v/v of Syzygium cumini leaf extract as obtained from (h) to each of the mixture as obtained from (i) under continuous stirring for a time period ranging from 4 to 8 h at a temperature in the range of 20-30° C.;
k) centrifuging the mixture as obtained in (i) to obtain the nanobiocomposite formulation (NCs).
3. The process as claimed in claim 2 , wherein the bleaching agent is selected from the group consisting of sodium chlorite, ammonium persulphate, and hydroxide peroxide.
4. The process as claimed in claim 2 , wherein the acid is selected from the group consisting of sulphuric acid, nitric acid, hydrochloric acid, and a mixture thereof.
5. The process as claimed in claim 2 , wherein the base is selected from the group consisting of potassium hydroxide and sodium hydroxide.
6. The process for the preparation of the nanobiocomposite formulation as claimed in claim 2 , wherein a pellet of the NCs is directly mixed with petrolatum base in the ratio of 1:1 to obtain the ointment form.
7. A process for treating a wound in a subject in need thereof, the process comprising topically applying the NCs of claim 1 to the wound.
8. The process of claim 7 , wherein the wound is exudating.