Device and method for making discrete volumes of a first fluid in contact with a second fluid, which are immiscible with each other
A method for generating, within a conduit, discrete volumes of one or more fluids that are immiscible with a second fluid. The discrete volumes can be used for biochemical or molecular biology procedures involving small volumes, for example, microliter-sized volumes, nanoliter-sized volumes, or smaller. The discrete volumes are separated from one another by a liquid that is immiscible with the fluid(s) of the discrete volumes, for example, aqueous immiscible-fluid-discrete volumes separated by an oil.
1. A method comprising:
suctioning discrete volumes of aqueous fluid and a spacing liquid into a first conduit such that the discrete volumes of aqueous fluid are carried and separated by the spacing liquid, the spacing liquid being disposed between the discrete volumes of aqueous fluid in the first conduit, and the spacing liquid being immiscible with the discrete volumes of aqueous fluid;
transferring a first set and a second set of the discrete volumes of aqueous fluid separated by the spacing liquid from the first conduit to a second conduit;
transferring the first set of discrete volumes of aqueous fluid separated by the spacing liquid from the second conduit to a third conduit;
processing the first set of discrete volumes of aqueous fluid in the third conduit;
holding in the second conduit the second set of the discrete volumes of aqueous fluid transferred to the second conduit while the first set of discrete volumes of aqueous fluid is transferred to and processed in the third conduit;
after holding in the second conduit, transferring the second set of discrete volumes of aqueous fluid from the second conduit to the third conduit; and
processing the second set of discrete volumes of aqueous fluid in the third conduit.
2. The method according to claim 1 , wherein the processing comprises thermally cycling the first set and the second set of discrete volumes of aqueous fluid in a thermal cycling nucleic acid sequence amplification process.
3. The method according to claim 2 , wherein the processing further comprises detecting a fluorescence of the first set and the second set of discrete volumes of aqueous fluid in a fluorescence detection process.
4. The method according to claim 1 , wherein the processing comprises detecting a fluorescence of the first set and the second set of discrete volumes of aqueous fluid in a fluorescence detection process.
5. The method according to claim 1 , wherein the discrete volumes of aqueous fluid and the spacing liquid are suctioned from a vessel into the first conduit.
6. The method according to claim 1 , wherein suctioning the discrete volumes of aqueous fluid and the spacing liquid into the first conduit comprises filling an inner cross-sectional area of the first conduit with the spacing liquid disposed between discrete volumes of aqueous fluid.