Protease variants having an improved washing performance
The disclosure relates to proteases, the amino acid sequence of which has been modified in particular with regard to the use thereof in detergents and cleaning agents, all sufficiently similar proteases having a corresponding modification and nucleic acids coding for them. The disclosure further relates to methods and uses of said proteases and to agents containing them, in particular detergents and cleaning agents.
1. A protease comprising a polypeptide having an amino acid sequence with at least 90% identity to SEQ ID NO: 1 or 2 and having an Asp residue at position 97 and a Glu residue at position 99 wherein numbering is according to SEQ ID NO: 1.
2. The protease according to claim 1 , having the sequence identical to that of SEQ ID NO. 6.
3. The protease according to claim 1 , having the sequence identical to that of SEQ ID NO. 9.
4. A method for producing a protease, the method comprising the step of:
introducing amino acid substitutions N97D and R99E in the numbering according to SEQ ID NO. 1 into a starting protease identical to the amino acid sequence specified in SEQ ID NO. 1 over the total length thereof to an extent of at least 90%; or
introducing amino acid substitutions S97D and S99E in the numbering according to SEQ ID NO. 1 into a starting protease identical to that specified in SEQ ID NO. 2 over the total length thereof to an extent of at 90%.