Method for producing a composition for treating a tooth lesion
The invention relates to a method for preparing a composition for treating a tooth lesion, said composition comprising peptides that are capable of undergoing self-assembly at a certain pH. The compositions of the invention are highly suitable for being used in the medical field, in particular for remineralising a tooth lesion such as a subsurface caries lesion.
1. A composition in lyophilized undissolved form comprising
a peptide that undergoes self-assembly at a pH value below 7.5, wherein the peptide is predominantly in a monomeric form;
a non-reducing sugar; and
a non-volatile buffer;
wherein upon reconstitution of the composition in water, a solution with an ionic strength of less than 0.15 and a pH that is 0.1 to 0.5 pH units above the pH at which the peptide starts to undergo self-assembly is obtained in which the peptide is predominantly present in a monomeric form.
2. The composition of claim 1 , wherein said peptide comprises the sequence of SEQ ID NO:3.
3. The composition of claim 1 , wherein said peptide comprises the sequence of SEQ ID NO: 4 or SEQ ID NO: 5.
4. The composition of claim 1 , wherein said peptide comprises the sequence depicted in SEQ ID NO:1 or SEQ ID NO:2 or a sequence having at least 80% sequence identity thereto.
5. The composition of claim 1 , wherein at least 70% of the peptide are present in monomeric form.
6. The composition of claim 1 , wherein at least 80% of the peptide are present in monomeric form.
7. The composition of claim 1 , wherein at least 90% of the peptide are present in monomeric form.