Device and method for preparing tissue, particularly adipose tissue
A device and a method for preparing adipose tissue for transplantation from lobular fat extracted, for instance by liposuction, the fat including a fluid component that have an oily component, a blood component and/or sterile solutions, and a solid component including cell fragments, cells and one or more cell macroagglomerates of heterogeneous size, wherein the device includes at least one washing and separating container having a washing chamber, the container has an inlet and an outlet for the liposuctioned material to enter the washing chamber through the inlet and for at least part of the material to exit the washing chamber through the outlet, the washing chamber including stirring means for forming an emulsion of fluid components.
1. A method of treating injuries or diseases in a patient comprising:
extracting lipoaspirate from donor areas of a first patient;
treating said lipoaspirate with a size reducing means for reducing the size of cell magroagglomerates of the lipoaspirate to averagely equal smaller cell agglomerates, wherein said size reducing means consist of at least one series of parallel or intersecting sheets or cutting wires, to form at least one size reducing net, through which the lipoaspirate is passed; and
injecting the treated material into a second patient,
wherein the step of injecting the treated material into the second patient is carried out by introducing a needle or cannula, and wherein the step of introducing is performed several times to create a 3D net of tunnels in subcutaneous or muscular tissue, with very small amounts of cell material being introduced therein.
2. The method as claimed in claim 1 , wherein the first patient and the second patient are a same person.
3. The method as claimed in claim 1 , wherein the step of injecting is performed using a blunted-end sterile cannula having a diameter of less than 1 mm.
4. The method as claimed in claim 1 , wherein the step of injecting is performed using a pointed-end, blunted-end, helical, or spiral cannula ( 5 ), said cannula being introduced and extracted by a rotary motion, thereby allowing the cannula, and hence the material, to enter bodily tissue by a spiral motion.
5. A method of treating injuries or diseases in a patient comprising:
extracting biological material from donor areas of a first patient;
treating said biological material; and
injecting the treated material into a second patient,
wherein the treating step comprises one or more of a size reducing step of the extracted biological material or the step of washing and separating a liquid phase from a solid cell phase, said treating step being carried out using the following method:
injecting a sterile washing solution into a washing chamber ( 101 ) of a washing and separating container ( 1 ), said washing chamber ( 101 ) containing at least one stirring element ( 104 ) having a shape and size relative to the washing chamber ( 101 ) that allows an emulsion of fluid components when said washing and separating container ( 1 ) is subjected to stirring, a manual stirring action being sufficient;
stirring said washing and separating container ( 1 ) to facilitate the emulsion of the fluid components with an emulsion-forming system ( 104 );
arranging said washing and separating container ( 1 ) in a position that provides a stratification of the solid components on the emulsion, which constitutes fat contained in the washing chamber ( 101 ); and
injecting a clean washing solution through an inlet ( 102 ), discharging the emulsion of fluid components from the washing chamber ( 101 ) through an outlet ( 103 ) of the washing and separating container ( 1 ), and removing the emulsion of the fluid components by density gradient;
wherein the step of injecting the treated material into the second patient is carried out by introducing a needle or cannula, and wherein the step of introducing is performed several times to create a 3D net of tunnels in subcutaneous or muscular tissue, with very small amounts of cell material being introduced therein.
6. The method as claimed in claim 5 , wherein the first patient and the second patient are a same person.
7. The method as claimed in claim 5 , wherein the treated and injected material is other than adipose tissue.