Rapid real time multipoint procedure for optimizing sperm state for use in assisted reproductive technologies

1. A method for determining the maturation state of mammalian sperm in real time for use in an assisted reproductive technology (ART) said method comprising:

collecting a mammalian ejaculate using a collection device prewarmed to about 30° C. to 40° C.;

incubating the ejaculate at a temperature above about 4° C.;

assaying, by reacting an aliquot of the incubated ejaculate with an agent including at least one of an antibody Fc fragment or an antibody, wherein the agent is reactive with a biomarker indicative of sperm cell maturation state, and wherein the biomarker is an Fc receptor;

detecting in real-time the percent of cells in the aliquot that are positive for the biomarker during the incubation period to determine the maturation state, wherein positive cells are those sperm cells which produce a detectable positive reaction with the agent that is indicative of maturation state;

repeating the assaying step with successive aliquots of the incubated ejaculate at intervals during incubation and observing real-time changes in the maturation state,

correlating the real-time changes in the maturation state with fertility;

selecting an optimum time point for peak fertility;

stabilizing the ejaculate at about the optimum time point for peak fertility; and

administering the stabilized ejaculate to a female or oocyte.

2. The method of claim 1 , wherein the incubating step includes incubating the ejaculate at a temperature in the range of about 40° C. to above 4° C.

3. The method of claim 2 , wherein the mammal is bovine and the incubating step includes incubating the ejaculate at a temperature in the range of about 5° C. to 15° C.

4. The method of claim 1 , wherein at least one of:

the agent is a fluorescent-labeled antibody Fc fragment or antibody;

the antibody includes a primary antibody that reacts with the biomarker and a secondary antibody that is labeled with a fluorescent label;

the reaction optionally includes a stabilizer for the sperm cells;

the percent positive cells is determined by an antibody-based method, a dye-based method, or a motility-based and microscopy based procedure; and

the reaction produces fluorescence in connection with positive reaction to the biomarker indicative of sperm cell maturation state on a sperm cell.

5. The method of claim 4 , wherein the reaction includes a stabilizer for the sperm cells.

6. The method of claim 1 , wherein stabilizing comprises washing, adding a protective diluent or both.

7. The method of claim 6 , wherein the method further comprises making straws having a predetermined amount of sperm cells and freezing the straws for artificial insemination.

8. The method of claim 1 , wherein repeating the assaying step is done at intervals of from about 30 minutes to an hour.

9. A method for determining the optimal time point of mammalian sperm stabilization for peak fertility for use in an assisted reproductive technology (ART) including intrauterine insemination, in-vitro fertilization and intracytoplasmic sperm injection said method comprising:

collecting a mammalian ejaculate using a collection device prewarmed to about the body temperature of the mammal;

incubating the ejaculate at a temperature in the range of about 4° C. to room temperature;

assaying by reacting an aliquot of the incubated ejaculate with an antibody during the incubation step to determine the maturation state by detecting in real-time the percent positive cells in the aliquot, wherein positive cells are those sperm cells which produce a detectable positive reaction with the antibody that is indicative of maturation state, and the antibody is reactive with a biomarker indicative of sperm cell maturation state, and wherein the biomarker is at least one of Fc receptor or CD46;

repeating the assaying step with successive aliquots of the ejaculate, and observing real time changes in the maturation state;

correlating the real time changes in the maturation state with fertility;

selecting the optimum time point for peak fertility;

stabilizing the ejaculate at about the optimum time point for peak fertility; and

administering the stabilized ejaculate to a female or oocyte.