Biomarker for diagnosing vascular diseases and the uses thereof
Provided are a composition for diagnosing vascular disease including an agent measuring a level of interleukin 12 receptor β2 protein in the blood, and a kit for diagnosing vascular disease including the same. Further, provided is a method for diagnosing vascular disease, the method including the step of measuring a level of interleukin 12 receptor β2 protein in a blood sample separated from an individual suspected of having vascular disease. Furthermore, provided are a composition for preventing or treating vascular disease including an interleukin 12 receptor β2 activity inhibitor, and a method of screening a therapeutic agent for vascular disease, the method including the step of treating smooth muscle cells with a test agent for vascular disease treatment and measuring an expression level of interleukin 12 receptor β2.
1. A method for diagnosing vascular disease, the method comprising the steps of:
(a) collecting an extracellular vesicle derived from smooth muscle cells in a blood sample separated from an individual suspected of having vascular disease;
(b) specifically binding to platelet-derived growth factor receptor-β (PDGFRβ) on the extracellular vesicle with an antibody or aptamer that specifically binds PDGFRβ, thereby isolating the extracellular vesicle;
(c) measuring a level of interleukin 12 receptor β2 protein in the isolated extracellular vesicle; and
(d) comparing the level of interleukin 12 receptor β2 protein measured in step (c) with that in a sample of a normal control group,
wherein if the blood sample of the individual has a higher level of interleukin 12 receptor B2 protein than the sample of the normal control group the individual is diagnosed with vascular disease.
2. The method of claim 1 , wherein the method further comprises measuring a level of the extracellular vesicle derived from smooth muscle cells obtained from step (a) using an antibody or an aptamer specific to an extracellular vesicle marker.
3. The method of claim 2 , wherein the extracellular vesicle marker is CD81, CD9 or CD63.
4. The method of claim 1 , wherein the blood sample of step (a) is a plasma sample.
5. The method of claim 1 , wherein the vascular disease is atherosclerosis, in-stent restenosis, myocardial infarction, acute coronary syndrome, or unstable angina.