Bis-biotinylation tags
Multi-biotinylated reactants are provided which can be used in divalent complexes for various applications such as colocalization, labeling, immobilization, and purification. Methods for constructing, purifying, and using the bis-biotinylated reactants are also provided. In certain embodiments, two bis-biotinylated reactants are bound to a single streptavidin tetramer to provide a complex having a 1:1 stoichiometry with respect to the bis-biotinylated reactants.
1. A compound having the formula
wherein F is a reactive functional group, C is a core group, L 1 is a first linking group, L 2 is a second linking group, and B is a biotin group, wherein L 1 and L 2 are identical groups selected from the group consisting of:
2. The compound of claim 1 , wherein F is a carboxylic acid group or an amino group.
3. The compound of claim 1 , wherein F is an azide group or an alkyne group.
4. The compound of claim 1 , wherein C comprises a six-membered ring.
5. The compound of claim 4 , wherein C comprises a tri-substituted aromatic six-membered ring.
6. The compound of claim 4 , wherein C comprises a tri-substituted triazine.
7. The compound of claim 4 , wherein C comprises a tri-substituted cyclohexane ring.
8. The compound of claim 1 , wherein the compound is