Management of corn rootworm and other insect pests
Trap and/or refuge crops and methods of use in managing corn rootworm and other insect pests of maize are provided. Some methods involve using trap crops comprising plants with enhanced susceptibility to one or more insect pests to lure and kill insect pests. Other methods involve using refuge crops comprising plants with enhanced susceptibility to one or more insect pests to monitor insect pest populations in an area or to promote mating between insecticide resistant and insecticide non-resistant insects.
1. A method for reducing herbivory of a maize crop by corn rootworm, European corn borer, and/or Japanese beetle, the method comprising:
a. growing the maize crop in an area; and
b. planting a trap crop comprising one or more maize plants with enhanced susceptibility to herbivory by corn rootworm, European corn borer, and/or Japanese beetle due to a reduction in endogenous expression of Crw1 and/or Crw2, within, adjacent to, or within 2 kilometers of the maize crop;
wherein Crw1 is a NAC transcription factor gene corresponding to a wild-type Crw1 locus encoding a Crw1 protein having at least 97% sequence identity to the amino acid sequence set forth in SEQ ID NO: 3; and
further wherein Crw2 is a glycosyltransferase gene corresponding to a wild-type Crw2 locus encoding a Crw2 protein having at least 95% sequence identity to the amino acid sequence set forth in SEQ ID NO: 29.
2. The method of claim 1 , further comprising:
a) destroying said corn rootworm, European corn borer, and/or Japanese beetle present on the trap crop;
b) rotating the area of the trap crop in the next growing season to control the corn rootworm, European corn borer, and/or Japanese beetle in the next generation; or
c) tilling the soil prior to planting in the next growing season to control the corn rootworm, European corn borer, and/or Japanese beetle in the next generation.
3. The method of claim 1 , wherein said trap crop comprises one or more maize plants that comprise a transgene that interferes with the life cycle of the corn rootworm, European corn borer, and/or Japanese beetle.
4. The method of claim 3 , wherein said transgene produces dsRNA that is introduced to the corn rootworm, European corn borer, and/or Japanese beetle upon feeding on plants in the trap crop.
5. The method of claim 1 , wherein said trap crop is about 10% of the total area comprising the trap crop and maize crop.
6. The method of claim 1 , wherein said trap crop is harvested with the maize crop.