Antibody array for measuring a panel of amyloids
Described are assay methods, modules and kits useful in the detection, treatment and/or prevention of dementia and related conditions, including but not limited to Alzheimer's disease and mild cognitive disorders.
1. A method for diagnosing Alzheimer's-related dementia in a patient comprising:
(a) obtaining a test sample from a patient;
(b) measuring the levels of each biomarker of a panel in said test sample, the panel comprising Aβ 16, Aβ 17, Aβ 42, Aβ 40, Aβ 38, total tau, P-tau, and C-tau;
(c) conducting a PET scan of said patient's brain using a radioactive diagnostic agent to estimate plaque density in said patient;
(d) diagnosing from said measuring step and said estimation of plaque density the presence, and/or progression of Alzheimer's-related dementia in said patient; and
(e) administering a treatment regimen.
2. The method of claim 1 wherein said method further comprises measuring a level of at least one additional biomarker in said sample and determining from said level of said first biomarker and said level of said at least one additional biomarker the presence, and/or progression of Alzheimer's-related dementia in said patient.
3. The method of claim 2 wherein said method further comprises comparing said level of each biomarker of the panel and said at least one additional biomarker(s) in said sample to a level of each biomarker of the panel and said at least one additional biomarker(s) in a normal control sample and diagnosing the presence, and/or progression of Alzheimer's-related dementia in said patient based on said comparison.
4. The method of claim 2 wherein said at least one additional biomarker comprises Aγ 39, Aβ 37, Aβ 34, Aβ 43, or combinations thereof.
5. The method of claim 1 wherein said radioactive diagnostic agent is AMYVID™ (Florbetapir), Pittsburgh Compound B, or combinations thereof.
6. A method for monitoring the disease progression of and/or efficacy of a first treatment regimen for Alzheimer's-related dementia in a patient, said method comprising:
(a) obtaining two or more test samples from a patient undergoing said first treatment regimen for Alzheimer's-related dementia, wherein said two or more test samples include (i) a first test sample obtained from said patient at time t and (ii) at least one additional test sample obtained at time (t+n), wherein n is an integer greater than 1;
(b) measuring the level(s) of each biomarker of a panel in said two or more test samples, the panel comprising Aβ 16, Aβ 17, Aβ 42, Aβ 40, Aβ 38, total tau, P-tau, and C-tau;
(c) conducting a PET scan of said patient's brain using a radioactive diagnostic agent to estimate plaque density in said patient;
(d) determining from said level(s) of said panel of biomarkers and said estimation of plaque density the progression of and/or efficacy of said first treatment regimen in said patient; and
(e) administering a second treatment regimen, which is the same as, or different from, the first treatment regimen.
7. The method of claim 6 wherein said method further comprises measuring a level of at least one additional biomarker in said two or more test samples and determining from said level(s) of said first biomarker and said at least one additional biomarker the disease progression and/or efficacy of said first treatment regimen for Alzheimer's-related dementia in said patient.
8. The method of claim 7 wherein said method further comprises comparing said level(s) of each biomarker of the panel and said at least one additional biomarker(s) in said two or more test samples to a level(s) of each biomarker of the panel and said at least one additional biomarker(s) in a normal control sample and determining said disease progression and/or treatment efficacy based on said comparison.
9. The method of claim 7 wherein said at least one additional biomarker comprises Aβ 39, Aβ 37, Aβ 34, Aβ 43, or combinations thereof.
10. The method of claim 7 wherein said radioactive diagnostic agent is AMYVID™ (Florbetapir), Pittsburgh Compound B, or combinations thereof.
11. A method for detecting plaques in the brain of a patient, said method comprising:
(a) obtaining two or more test samples from a patient undergoing a first treatment regimen for dementia, wherein said two or more test samples include (i) a first test sample obtained from said patient at time t and (ii) at least one additional test sample obtained at time (t+n), wherein n is an integer greater than 1;
(b) measuring the level(s) of each biomarker of a panel in said two or more test samples, the panel comprising Aβ 16, Aβ 17, Aβ 42, Aβ 40, Aβ 38, Aβ 39, Aβ 37, Aβ 34, Aβ 43, total tau, P-tau, and C-tau;
(c) conducting a PET scan of said patient's brain using a radioactive diagnostic agent;
(d) determining from said level(s) of said panel of biomarkers and said PET scan the presence of plaques in the brain of said patient; and
(e) administering a second treatment regimen, which is the same as, or different from, the first treatment regimen.
12. The method of claim 11 wherein said method further comprises comparing said level(s) of each biomarker of said panel of biomarkers in said two or more test samples to a level of each of said biomarker in said panel of biomarkers in a normal control sample and determining the presence of plaques in the brain of said patient based on said comparison.