Method for preparing microbial preparation and microbial preparation produced by the same
The present disclosure relates to a method for preparing an aglycone or hydrolyzed glycoside converted from a glycoside and, specifically, to a method for preparing an aglycone or hydrolyzed glycoside from a glycoside by converting a glycoside into an aglycone form or hydrolyzed glycoside by using a microorganism producing β-glycosidase, and then recovering the aglycone or hydrolyzed glycoside accumulated in the cells of the microorganism.
1. A method of preparing a microorganism preparation showing a high body absorption rate of a converted ginsenoside, in which the converted ginsenoside is accumulated in cells, comprising accumulating the converted ginsenoside in the microorganism by incubating the microorganism expressing β-glycosidase in a medium containing ginseng extracts, wherein a concentration of the converted ginsenoside accumulated in the cells of the microorganism is more than 2 times higher than that of the converted ginsenoside in the medium.
2. The method according to claim 1 , wherein the converted ginsenoside is selected from the group consisting of rg3, rc, and f2, and the ginseng extracts comprise one or more of a ginsenoside selected from the group consisting of rg1 and rb 1.
3. The method according to claim 1 , wherein the microorganism expressing β-glycosidase is one or more selected from the group consisting of lactic acid bacteria, bifidus, yeast, corynebacterium, aspergillus and clostridium.
4. The method according to claim 3 , wherein the lactic acid bacteria is one or more selected from the group consisting of L. plantarum, L. sakei, L. rhamnosus GG, L. delbrueckii, L. acidophilus, L. johnsonii, L. casei and L. gasser.
5. The method according to claim 3 , wherein the lactic acid bacteria is Lactobacillus plantarum K8 (accession number: KCTC 10887BP).
6. The method according to claim 1 , wherein the microorganism producing the β-glycosidase is one or more selected from the group consisting of generally recognized as safe (GRAS) microorganisms, bifidus, yeast, Bacillus licheniformis, S. thermophilus, L. casei, Streptomyces sp. Bifidobacteria, Lactobacillus delbrueckii Rh2, Sporosarcina sp., Saccharomyces cerevisiae, Pyrococcus furiosus, Lactobacillus plantarum, Aspergillus ochraceus, Aactobacillus delbrueckii Rh2, Pseudomonas sp., Aspergillus niger, Pseudomonas fluorescens, Bifidobacterium adolescentis, Aspergillus sojae, Cunninghamella blakesleeana, Cifidobacteria and Lactobacillus, Lactic acid bacteria, Bifidobacterium pseudocatenulatum, Penicillium melinii, Eubacterium ramulus, Clostridium orbiscindens, Aspergillus awamori, Lactobacillus brevis, Aspergillus parasiticus speare BGB, Aspergillus aculeatus , and Aspergillus niger.
7. The method according to claim 1 , wherein the incubating the microorganism comprises steps of static culture for microbial activation, shaking culture, and feeding culture in sequence.
8. The method according to claim 7 , wherein the static culture is performed for 12 hrs at 37° C.
9. The method according to claim 7 , wherein the shaking culture is performed under a condition of 6 rpm for 12 hrs at 37° C.
10. The method according to claim 1 , wherein the incubating further comprises inoculating and then incubating the microorganism in a medium, is followed by transferring to a minimal medium comprising a glycoside and then culturing the microorganism additionally for 1 to 36 hrs.