Systems and methods for electrochemical ketone detection and measurement
A system for the electrochemical detection of ketone levels includes a test strip including an electrode and a counter electrode, the electrode and counter electrode located in a sample reception area. The system further includes a coating on one of the electrode and counter electrode, the coating including a mediator for ketones. Optionally, the mediator is ferricyanide.
1. A system for the electrochemical detection of ketone and glucose levels, the system comprising:
a test strip including a first electrode and a first counter electrode and a second electrode and a second counter electrode, the first electrode and first counter electrode located in a first sample reception area and the second electrode and second counter electrode located in a second sample reception area, the first and second sample reception areas interconnected via an inlet, each of the first and second sample reception areas being separate; and
a first coating on one of the first electrode and first counter electrode, the first coating including a mediator for ketones, wherein the first coating additionally includes diaphorase and the first coating includes 1-Methoxy-5-methylphenazinium methylsulfate;
a second coating on one of the second electrode and second counter electrode, the second coating including a mediator for glucose; wherein the mediator for ketones is ferricyanide, the first coating additionally includes hydroxybutyrate dehydrogenase, and nicotinamide adenine dinucleotide (NAD) and wherein the second coating includes 1-Methoxy-5-methylphenazinium methylsulfate and the second coating does not include diaphorase; and
a meter engaging the test strip having an electrical source configured to provide different startup times for the first electrode and first counter electrode and the second electrode and second counter electrode.
2. The system of claim 1 , wherein the first coating includes a buffer and a surfactant.
3. The system of claim 1 , wherein the first and second sample reception areas form respective first and second sample chambers separated from each other.
4. The system of claim 3 , wherein the first and second sample chambers are separated by a dividing wall.
5. A system for the electrochemical detection of ketone and glucose levels, the system comprising:
a test strip including a first electrode and a first counter electrode and a second electrode and a second counter electrode, the first electrode and first counter electrode located in a first sample chamber, the second electrode and second counter electrode located in a second sample chamber, the first and second sample chambers divided by a dividing wall and interconnected via an inlet;
a first coating on one of the first electrode and first counter electrode, the first coating including a mediator for ketones and including hydroxybutyrate dehydrogenase, 1-Methoxy-5-methylphenazinium methylsulfate and nicotinamide adenine dinucleotide (NAD), wherein the mediator for ketones is ferricyanide; and
a second coating on one of the second electrode and second counter electrode, the second coating including a mediator for glucose, and wherein the second coating includes 1-Methoxy-5-methylphenazinium methylsulfate and the second coating does not include diaphorase.
6. The system of claim 5 , further comprising a meter engaging the test strip, the meter having an electrical source configured to provide different startup times for the first electrode and first counter electrode and the second electrode and second counter electrode.