Anti-serum albumin binding variants
The invention relates to improved variants of the anti-serum albumin immunoglobulin single variable domain DOM7h-11, as well as ligands and drug conjugates comprising such variants, compositions, nucleic acids, vectors and hosts.
1. An anti-serum albumin (SA) immunoglobulin single variable domain comprising the amino acid sequence of DOM7h-11-15 (SEQ ID NO:2), but for the replacement of arginine at position 108 thereof by cysteine.
2. A multispecific ligand comprising an the anti-SA immunoglobulin single variable domain of claim 1 and a binding moiety that specifically binds a target antigen other than SA.
3. The anti-serum albumin (SA) immunoglobulin single variable domain of claim 1 , wherein the variable domain is conjugated to an NCE drug.
4. A composition comprising the anti-serum albumin (SA) immunoglobulin single variable domain of claim 1 and a pharmaceutically acceptable diluent, carrier, excipient or vehicle.
5. A nucleic acid comprising a nucleotide sequence encoding the anti-serum albumin (SA) immunoglobulin single variable domain of claim 1 .
6. A vector comprising the nucleic acid of claim 5 .
7. An isolated host cell comprising the vector of claim 6 .