Biological method for preparing heme iron not derived from porcine blood
The present invention relates to heme iron not derived from porcine blood and a method of preparing the same, and more particularly to a method of biologically preparing heme iron not derived from porcine blood, a method of preparing a salt thereof, and an iron supplement containing the salt thus prepared as an active ingredient.
1. A method of biologically preparing a salt of heme iron of Chemical Formula 1 below, not derived from porcine blood and containing no animal component, the method comprising:
A) producing heme iron by culturing Escherichia coli DH5α pLEX_HMD (Accession number: KCTC 13173BP) at 39 to 44° C. and a pH of 7 to 9 in a medium containing no animal component;
B) purifying the heme iron produced in step A); and
C) preparing a salt of the heme iron purified in step B)
2. The method of claim 1 , wherein the medium includes plant-derived peptone and a yeast extract prepared from yeast cultured in a plant-derived medium.
3. The method of claim 1 , wherein the pH is adjusted using succinic acid.
4. The method of claim 1 , wherein step B) is performed through filtration process using diatomaceous earth.
5. The method of claim 4 , wherein the diatomaceous earth is Celite®545.