Recombinant yeast producing 3-hydroxypropionic acid and method for producing 3-hydroxypropionic acid using the same

1. A recombinant yeast selected from Saccharomyces cerevisiae, Kazachstania exigua, Kazachstania bulderi , and Candida humilis and comprising an active 3-hydroxypropionic acid (3-HP) biosynthetic pathway of [Pyruvate→Acetaldehyde→Acetyl-CoA→Malonyl-CoA→Malonate semialdehyde→3-HP], wherein the yeast comprises:

an exogenous gene encoding acetylating acetaldehyde dehydrogenase (AADH), which converts acetaldehyde directly to acetyl-CoA and that has an amino acid sequence of at least 95% sequence identity to SEQ ID NO: 164;

an endogenous or exogenous gene encoding an acetyl-CoA carboxylase (ACC) that has an amino acid sequence of at least 95% sequence identity to SEQ ID NO: 101;

an exogenous gene encoding a malonyl-CoA reductase (MCR) that has an amino acid sequence of at least 95% sequence identity to SEQ ID NO: 203; and

an exogenous gene encoding an hydroxypropionate dehydrogenase (HPDH) that has an amino acid sequence of at least 95% sequence identity to SEQ ID NO: 236.

2. A method of preparing 3-hydroxypropionic acid (3-HP) comprising:

(a) culturing the recombinant yeast of claim 1 in a medium including at least one carbon source, thereby producing 3-HP; and

(b) isolating 3-HP from the culture.

3. The method of preparing 3-HP of claim 2 , wherein the carbon source is one or more selected from the group consisting of glucose, xylose, arabinose, sucrose, fructose, galactose, cellulose, glucose oligomers and glycerol.

4. The method of preparing 3-HP of claim 2 , wherein culturing is performed at a pH in the range of 2.6 to 4.0.