Method of generating collagen fibers
A method of generating a collagen fiber is disclosed. Fibers generated thereby are also disclosed as well as scaffolds comprising such fibers.
1. A method of generating a collagen fiber, the method comprising:
(a) extruding a solution of collagen into a coagulating solution to generate the collagen fiber; and
(b) drawing the fiber in said coagulating solution, wherein the rate of said drawing of said fiber is higher than the rate of extruding said solution of collagen into said coagulating solution, wherein the rate of said drawing of said fiber is at least three times higher than the rate of extruding said solution of collagen into said coagulating solution.
2. The method of claim 1 , further comprising isolating the collagen fiber following said extruding.
3. The method of claim 2 , further comprising drying the collagen fiber following said isolating.
4. The method of claim 1 , wherein said extruding is effected concomitantly with said drawing.
5. The method of claim 3 , further comprising polymerizing said collagen following said extruding.
6. The method of claim 3 , further comprising crosslinking said collagen following said extruding.
7. The method of claim 6 , wherein said crosslinking is effected in said coagulating solution.
8. The method of claim 1 , wherein said extruding is effected using a spinneret.
9. The method of claim 1 , wherein said extruding is effected by passing said collagen solution through an orifice comprising an inner diameter between 10 μm-100 μm.
10. The method of claim 1 , wherein said collagen comprises recombinant atelocollagen.
11. The method of claim 1 , wherein said collagen is human collagen.
12. The method of claim 1 , wherein said collagen is present at a concentration between 20-200 mg/ml in said solution.
13. The method of claim 1 , wherein said collagen is present at a concentration between 30-70 mg/ml in said solution.
14. The method of claim 13 , wherein said extruding is effected by passing through an orifice comprising an inner diameter of about 30 μm.
15. The method of claim 1 , wherein said solution of collagen is an acidic solution.
16. A collagen fiber produced by the method of claim 1 .
17. The collagen fiber of claim 16 , having a diameter of about 10 μm.
18. The collagen fiber of claim 16 , having a tensile stress at break of between 60-200 MPa when wet.
19. The collagen fiber of claim 16 , having a Young's modulus of between 500-1200 MPa when wet.
20. The collagen fiber of claim 16 , having a strain at break of between 0.15-0.3 when wet.
21. A scaffold comprising the collagen fiber of claim 16 .
22. The scaffold of claim 21 , further comprising a cell population seeded thereon.
23. A pharmaceutical composition comprising the isolated collgaen fiber of claim 2 .
24. A cosmetic composition comprising the isolated collagen fiber of claim 2 .