Biosensing systems and methods using a FET
Systems and methods for sensing analytes using an extended gate field effect transistor (EGFET) are provided. A biosensing system can utilize a biodetection layer on a substrate, which can be coupled to a field effect transistor (FET). The coupling can be such that the gate of the field effect transistor is connected to the substrate having the biodetection layer thereon. The functionalized substrate can include a well-defined area that can hold a specific, pre-determined volume of fluid on top of it. An external electrode can be dipped in the fluid and can then be connected to a power source supplying a gate voltage. The presence or concentration of the target analyte in the fluid can be determined based on the source-drain characteristics of the FET.
1. A biosensing system for detecting a target biological analyte, the system comprising:
a field effect transistor (FET) comprising a gate, a source, and a drain;
an extended gate comprising a conductive substrate in electrical contact with the gate of the FET; and
an external electrode that is not attached to the FET or the extended gate, configured to apply a gate voltage to a fluid disposed on a predefined area of the conductive substrate,
the conductive substrate comprising a screen printed carbon electrode (SPCE) comprising a reference electrode and a working electrode,
the extended gate being a single, continuous extended gate, and
the reference electrode and the working electrode both being disposed on a same surface of the single continuous extended gate.
2. The biosensing system according to claim 1 , the conductive substrate being a molecular imprinted polymer (MIP) that is specifically functionalized for the target biological analyte.
3. The biosensing system according to claim 1 , the target biological analyte being cortisol.
4. The biosensing system according to claim 1 , the external electrode being connected to a gate voltage source,
the gate voltage source being connected to the reference electrode of the SPCE, and
the gate of the FET being connected to the working electrode of the SPCE.
5. The biosensing system according to claim 1 , the gate of the FET being connected to the working electrode of the SPCE.
6. The biosensing system according to claim 1 , the drain of the FET being connected to a drain voltage source, and the source of the FET being grounded.
7. The biosensing system according to claim 1 , the source of the FET being connected to a source voltage source, and the drain of the FET being grounded.
8. The biosensing system according to claim 1 , the predefined area of the conductive substrate comprising a well in the conductive substrate configured to receive the fluid.
9. The biosensing system according to claim 1 , further comprising:
a small current amplifier in operable communication with the FET; and
a microcontroller in operable communication with the small current amplifier,
the microcontroller being configured to receive a source-drain characteristic and determine a concentration of the target biological analyte based on the source-drain characteristic, and
the source-drain characteristic comprising at least one of a source-drain current and a source-drain voltage.
10. A method of sensing a concentration of a target biological analyte, the method comprising:
providing a fluid comprising the target biological analyte to an extended gate comprising a single, continuous conductive substrate in electrical contact with the gate of a field effect transistor (FET), the FET comprising the gate, a source, and a drain;
dipping an external electrode, which is not attached to the FET or the extended gate, in the fluid;
providing a gate voltage to the external electrode via a gate voltage source connected to the external electrode;
analyzing, by a controller in operable communication with the FET, a source-drain characteristic of the FET while the gate voltage is provided to the external electrode; and
determining the concentration of the target biological molecule based on the source-drain characteristic of the FET,
the source-drain characteristic comprising at least one of a source-drain current and a source-drain voltage,
the conductive substrate comprising a screen printed carbon electrode (SPCE) comprising a reference electrode and a working electrode,
the extended gate being a single, continuous extended gate, and
the reference electrode and the working electrode both being disposed on a same surface of the single continuous extended gate.
11. The method according to claim 10 , the conductive substrate being a molecular imprinted polymer (MIP) that is specifically functionalized for the target biological analyte.
12. The method according to claim 10 , the target biological analyte being cortisol.
13. The method according to claim 10 , the gate voltage source being connected to the reference electrode of the SPCE, and
the gate of the FET being connected to the working electrode of the SPCE.
14. The method according to claim 10 , the drain of the FET being connected to a drain voltage source, and the source of the FET being grounded.
15. The method according to claim 10 , the source of the FET being connected to a source voltage source, and the drain of the FET being grounded.
16. The method according to claim 10 , the fluid being provided to a predefined area of the conductive substrate, the predefined area comprising either: a) a well in the conductive substrate in which the fluid is deposited; or b) a flat area on an upper surface of the conductive substrate on which the fluid is deposited.
17. The method according to claim 10 , in which a small current amplifier is in operable communication with the FET,
the controller being a microcontroller in operable communication with the small current amplifier.
18. A method of sensing a concentration of a target biological analyte, the method comprising:
providing a fluid comprising the target biological analyte to an extended gate comprising a single, continuous conductive substrate in electrical contact with the gate of a field effect transistor (FET), the FET comprising the gate, a source, and a drain;
dipping an external electrode, which is not attached to the FET or the extended gate, in the fluid;
providing a gate voltage to the external electrode via a gate voltage source connected to the external electrode;
analyzing, by a controller in operable communication with the FET, a source-drain characteristic of the FET while the gate voltage is provided to the external electrode; and
determining the concentration of the target biological molecule based on the source-drain characteristic of the FET,
the source-drain characteristic comprising at least one of a source-drain current and a source-drain voltage,
the conductive substrate being a molecular imprinted polymer (MIP) that is specifically functionalized for the target biological analyte,
the target biological analyte being cortisol,
the conductive substrate comprising a screen printed carbon electrode (SPCE) comprising a reference electrode and a working electrode,
the gate voltage source being connected to the reference electrode of the SPCE,
the gate of the FET being connected to the working electrode of the SPCE,
the fluid being provided to a predefined area of the conductive substrate, the predefined area comprising either: a) a well in the conductive substrate in which the fluid is deposited; or b) a flat area on an upper surface of the conductive substrate on which the fluid is deposited,
the controller being a microcontroller in operable communication with a small current amplifier that is in operable communication with the FET
the extended gate being a single, continuous extended gate, and
the reference electrode and the working electrode both being disposed on a same surface of the single, continuous extended gate.