Extraction of cfDNA from biological samples
Focused acoustic treatment of samples including a target biomolecule, such as cfDNA, may aid in the recovery of the biomolecule from a sample. cfDNA in a sample, whether chemically stabilized or not, may be linked or otherwise bound to histones or other proteins, e.g., by hydrogen bonds of histones or chaperone proteins to DNA and/or covalent crosslinks of such proteins to the DNA. Focused acoustic energy may remove or disrupt such links, aiding in isolation of the cfDNA from the sample.
1. A method for processing a sample containing a biomolecule to be recovered from the sample, comprising:
providing an initial sample including cfDNA and whole cells;
separating cfDNA from the whole cells in the initial sample to provide a sample containing cfDNA;
providing the sample containing cfDNA that has histones and/or other proteins bound to the cfDNA in an acoustic treatment vessel;
exposing the sample in the vessel to focused acoustic energy specifically adapted to remove or disrupt bonds between the cfDNA and bound histones and/or other proteins such that the specifically adapted focused acoustic energy alone acts to remove or disrupt bonds between the cfDNA and bound histones and/or other proteins without shearing of the cfDNA; and
extracting the cfDNA from the sample after bonds between the cfDNA and bound histones and/or other proteins have been removed or disrupted by the focused acoustic energy;
wherein the focused acoustic energy has a duty cycle of at least 5%, a 10 W to 80 W Peak Incident Power (PIP), and 50 cycles per burst for a processing time of 5 seconds to 120 seconds per sample.
2. The method of claim 1 , wherein the sample containing cfDNA is previously chemically stabilized prior to being provided in the acoustic treatment vessel.
3. The method of claim 1 , wherein the sample is a portion of a blood sample.
4. The method of claim 3 , wherein the blood sample is chemically stabilized with a fixative.
5. The method of claim 3 , wherein the sample includes serum separated from the blood sample by centrifugation.
6. The method of claim 1 , wherein the acoustic treatment vessel is positioned in a fluid coupling medium that transmits the focused acoustic energy to the vessel.
7. The method of claim 1 , wherein the step of exposing the sample in the vessel to focused acoustic energy includes moving a focal zone of the acoustic energy relative to the sample, and wherein a volume of the focal zone is less than a volume of the sample.
8. The method of claim 1 , wherein the step of exposing the sample in the vessel to focused acoustic energy includes maintaining a focal zone of the acoustic energy stationary relative to the sample, and wherein a volume of the focal zone is larger than a volume of the sample.
9. The method of claim 1 , wherein the sample is not chemically stabilized.
10. The method of claim 1 , wherein the step of exposing enhances a reverse crosslinking rate of histones to the cfDNA.
11. The method of claim 1 , wherein the step of exposing disrupts hydrogen bonding of histones or chaperone proteins to the cfDNA.
12. The method of claim 1 , wherein bonds between cfDNA and bound histones and/or other proteins were created by chemical stabilization.
13. The method of claim 1 , wherein the sample includes blood, spinal fluid, saliva, sputum, stool or urine.
14. The method of claim 1 , wherein the sample is treated with a stabilization compound including a formaldehyde-providing compound or a disaccharide prior to being provided in the acoustic treatment vessel.
15. The method of claim 1 , wherein the focused acoustic energy is generated by an acoustic energy source spaced from and exterior to the vessel, and the focused acoustic energy comprises a frequency of between about 100 kilohertz and about 100 megahertz and a focal zone having a width of less than about 2 centimeters.
16. The method of claim 15 , wherein the acoustic energy source includes an acoustic transducer having a dome shape and arranged to generate focused acoustic energy to create the focal zone.
17. The method of claim 1 , wherein the sample has a volume of 10 microliters to 150 milliliters.