Real-time focusing in line scan imaging
Systems and methods for capturing a digital image of a slide using an imaging line sensor and a focusing line sensor. In an embodiment, a beam-splitter is optically coupled to an objective lens and configured to receive one or more images of a portion of a sample through the objective lens. The beam-splitter simultaneously provides a first portion of the one or more images to the focusing sensor and a second portion of the one or more images to the imaging sensor. A processor controls the stage and/or objective lens such that each portion of the one or more images is received by the focusing sensor prior to it being received by the imaging sensor. In this manner, a focus of the objective lens can be controlled using data received from the focusing sensor prior to capturing an image of a portion of the sample using the imaging sensor.
1. A system for capturing a digital image of a slide, the system comprising:
an objective lens having a single optical axis;
a motorized positioner to control the objective lens;
a stage configured to support a sample;
at least one line sensor;
a moveable wedge prism configured to move into and out of a field of view of the at least one line sensor; and
at least one processor that controls the moveable wedge prism and one or more of the stage and the objective lens such that each portion of one or more images of the sample is received by the at least one line sensor, both while the moveable wedge prism is in the field of view and while the moveable wedge prism is not in the field of view.
2. The system of claim 1 , wherein the stage is a motorized stage, and the at least one processor controls the motorized stage.
3. The system of claim 1 , wherein the at least one processor further:
receives a captured first portion of the one or more images from the at least one line sensor while the moveable wedge prism is in the field of view;
determines a focus height for the captured first portion of the one or more images; and
causes the objective lens to move to the focus height prior to the first portion of the one or more images being captured by the at least one line sensor while the moveable wedge prism is not in the field of view.
4. The system of claim 1 , wherein the at least one line sensor comprises two or more line sensors.
5. The system of claim 1 , wherein the at least one processor further aligns adjacent portions of the one or more images into a single digital image of at least a portion of the sample.
6. A method of capturing a digital image of a slide, the method comprising:
by an objective lens having a single optical axis, acquiring one or more images of at least a portion of a sample supported on a stage;
and
by at least one processor, controlling a moveable wedge prism and one or more of the stage and the objective lens such that each portion of the one or more images is received by at least one line sensor, both while the moveable wedge prism is in a field of view of the at least one line sensor and while the moveable wedge prism is not in the field of view.