Compositions comprising recombinant
View Patent ↗The present invention relates to a composition comprising a) recombinant exosporium-producing Bacillus cells that express a fusion protein comprising: (i) at least one plant growth stimulating protein or peptide and (ii) a targeting sequence that localizes the fusion protein to the exosporium of the Bacillus cells; and b) at least one particular insecticide disclosed herein in a synergistically effective amount. Furthermore, the present invention relates to the use of this composition as well as a method for enhancing plant growth, promoting plant health, and/or reducing overall damage of plants and plant parts.
1. A composition comprising:
(a) recombinant exosporium-producing Bacillus cells of a Bacillus cereus family member that express a fusion protein comprising:
(i) an aminocyclopropane-1-carboxylic acid deaminase; and
(ii) a targeting sequence, or an exosporium protein, wherein the targeting sequence or the exosporium protein comprises:
an amino acid sequence having at least 43% sequence identity with amino acids 20-35 of SEQ ID NO: 1, wherein the sequence identity with amino acids 25-35 of SEQ ID NO: 1 is at least 54%;
a targeting sequence comprising amino acids 1-35 of SEQ ID NO: 1;
a targeting sequence comprising amino acids 20-35 of SEQ ID NO: 1;
a targeting sequence comprising amino acids 22-31 of SEQ ID NO: 1;
a targeting sequence comprising amino acids 22-33 of SEQ ID NO: 1;
a targeting sequence comprising amino acids 20-31 of SEQ ID NO: 1;
a targeting sequence comprising SEQ ID NO: 1; or
an exosporium protein comprising an amino acid sequence having at least 85% sequence identity with SEQ ID NO: 2 and
(b) at least one insecticide selected from the group consisting of abamectin, carbofuran, cyazypyr, cycloaprid, spinosad, sulfoxaflor, and tefluthrin, in a synergistically effective amount.
2. The composition of claim 1 , wherein the recombinant Bacillus cells are from Bacillus thuringiensis BT013A.
3. The composition according to claim 1 , wherein the at least one insecticide is spinosad.
4. The composition according to claim 1 , wherein the at least one insecticide is abamectin.
5. The composition according to claim 1 , wherein the at least one insecticide is carbofuran.
6. A seed treated with the composition according to claim 1 .
7. A method of treating a plant, a plant part, or the locus surrounding the plant to enhance plant growth and/or promote plant health comprising the step of simultaneously or sequentially applying:
a) recombinant exosporium-producing Bacillus cells of a Bacillus cereus family member that express a fusion protein comprising:
(i) an aminocyclopropane-1-carboxylic acid deaminase; and
(ii) a targeting sequence, or an exosporium protein, wherein the targeting sequence or the exosporium protein comprises:
an amino acid sequence having at least 43% sequence identity with amino acids 20-35 of SEQ ID NO: 1, wherein the sequence identity with amino acids 25-35 of SEQ ID NO: 1 is at least 54%;
a targeting sequence comprising amino acids 1-35 of SEQ ID NO: 1;
a targeting sequence comprising amino acids 20-35 of SEQ ID NO: 1;
a targeting sequence comprising amino acids 22-31 of SEQ ID NO: 1;
a targeting sequence comprising amino acids 22-33 of SEQ ID NO: 1;
a targeting sequence comprising amino acids 20-31 of SEQ ID NO: 1;
a targeting sequence comprising SEQ ID NO: 1; or
an exosporium protein comprising an amino acid sequence having at least 85% sequence identity with SEQ ID NO: 2 and
(b) at least one insecticide selected from the group consisting of abamectin, carbofuran, cyazypyr, cycloaprid, spinosad, sulfoxaflor, and tefluthrin, in a synergistically effective amount.