Therapeutic microbiota for the treatment and/or prevention of food allergy
Disclosed are methods and compositions for the prevention and treatment of food allergy. In particular, described herein are microbial consortia, including minimal microbial consortia, that can prevent and/or cure food allergy. In certain embodiments, the consortia comprise certain members of the taxa Clostridiales and/or Bacteroidetes.
1. A pharmaceutical composition comprising:
(i) a purified mixture of live and isolated bacteria for treatment of an inflammatory disorder in an individual in need thereof, wherein the purified mixture of live and isolated bacteria comprises Clostridium bifermentans, Clostridium hiranonis, Clostridium ramosum, Clostridium scindens, Clostridium leptum , and Clostridium sardiniensis ; and
(ii) a pharmaceutically acceptable carrier, wherein the pharmaceutical composition is formulated for viable delivery to the intestines via oral administration.
2. The pharmaceutical composition of claim 1 , wherein the pharmaceutical composition is formulated as a suspension, and wherein the suspension is an aqueous or oily suspension.
3. The pharmaceutical composition of claim 1 , wherein the inflammatory disorder is a food allergy.
4. The pharmaceutical composition of claim 1 , wherein the purified mixture of live and isolated bacteria comprises strains present in substantially equal biomass.
5. The pharmaceutical composition of claim 1 , wherein the purified mixture of live and isolated bacteria comprises strains present in an amount of at least 1×10 8 CFUs/ml.
6. The pharmaceutical composition of claim 1 , wherein the purified mixture of live and isolated bacteria comprises strains present in a total amount of at least 1×10 5 CFUs.
7. The pharmaceutical composition of claim 1 , wherein the purified mixture of live and isolated bacteria comprises strains present in a total amount of at least 1×10 8 CFUs.
8. The pharmaceutical composition of claim 1 , wherein the purified mixture of live and isolated bacteria does not comprise any of Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis, Enterobacter cloacae, Bilophila wadsworthia, Alistipes onderdonkii, Desulfovibrio species, Lactobacillus johnsonii , and Parasutterella excrementihominis.
9. The pharmaceutical composition of claim 1 , wherein the purified mixture of live and isolated bacteria comprises up to eleven bacterial strains in total.
10. The pharmaceutical composition of claim 1 , wherein the purified mixture of live and isolated bacteria consists of: Clostridium ramosum, Clostridium scindens, Clostridium hiranonis, Clostridium bifermentans, Clostridium leptum , and Clostridium sardiniensis.
11. A method, comprising orally administering to a subject having an inflammatory disorder a pharmaceutical composition of claim 1 comprising:
(i) a purified mixture of live and isolated bacteria for treatment of an inflammatory disorder in an individual in need thereof, wherein the purified mixture of live and isolated bacteria comprises Clostridium bifermentans, Clostridium hiranonis, Clostridium ramosum, Clostridium scindens, Clostridium leptum , and Clostridium sardiniensis ; and
(ii) a pharmaceutically acceptable carrier, wherein the pharmaceutical composition is formulated for viable delivery to the intestines.
12. The method of claim 11 , wherein pharmaceutical composition is formulated as a suspension and wherein the suspension is an aqueous or oily suspension.
13. The method of claim 12 , wherein the purified mixture of live and isolated bacteria comprises up to eleven strains of bacteria.
14. The method of claim 11 , wherein the purified mixture of live and isolated bacteria comprises strains present in substantially equal biomass.
15. The method of claim 11 , wherein the purified mixture of live and isolated bacteria comprises strains present in an amount of at least 1×10 8 CFUs/ml.
16. The method of claim 11 , wherein the purified mixture of live and isolated bacteria comprises strains present in a total amount of at least 1×10 5 CFUs.
17. The method of claim 11 , wherein the purified mixture of live and isolated bacteria comprises strains present in a total amount of at least 1×10 8 CFUs.
18. The method of claim 11 , wherein the inflammatory disorder is a food allergy.
19. The method of claim 18 , wherein the food allergy is an allergy to soy, wheat, eggs, dairy, peanuts, tree nuts, shellfish, fish, mushrooms, or stone fruits.
20. The method of claim 11 , wherein the purified mixture of live and isolated bacteria consists of: Clostridium ramosum, Clostridium scindens, Clostridium hiranonis, Clostridium bifermentans, Clostridium leptum , and Clostridium sardiniensis.