Compositions containing platelet contents
This document provides methods and materials relating to platelet lysates. For example, methods and materials for using platelet lysate compositions to grow adult stem cells, to differentiate adult stem cells, to grow primary cell cultures, to grow tumor stem cells, and to identify effective growth factors are provided.
1. A stable human platelet lysate composition comprising:
a lysed platelet preparation comprising:
i) plasma,
ii) a total protein concentration of at least about 35 mg per mL,
iii) a protein fraction that contains growth factor activity;
iv) greater than 200 pg human VEGF per mL; and
wherein said platelet lysate composition comprises protein complexes greater than 50 kDa.
2. The platelet lysate composition of claim 1 , wherein the composition further comprises: about 180 pg/mL FGF.
3. The platelet lysate composition of claim 1 , wherein the composition further comprises: about 54 ng/mL TGF-β.
4. The platelet lysate composition of claim 1 , wherein the composition further comprises: about 8.5 ng/mL PDGF-BB.
5. The platelet lysate composition of claim 1 , wherein the composition further comprises: about 180 pg/mL FGF; about 8.5 ng/mL PDGF; about 132 ng/mL IGF-1; and about 54 ng/mL TGF-β.
6. The platelet lysate composition of claim 1 , wherein the total protein concentration is least about 48 mg per mL.
7. The platelet lysate composition of claim 1 , wherein the platelet lysate composition is passed through a 0.20 μm or smaller filter after begin filtered by a 0.45 μm or smaller filter.
8. The platelet lysate composition of claim 1 , wherein the composition further comprises heparin.
9. The platelet lysate composition of claim 1 , wherein the composition is a spray, glue, or a suture.
10. The platelet lysate composition of claim 8 , wherein the composition is a spray, glue, or a suture.
11. The platelet lysate composition of claim 1 , wherein the protein fraction has a molecular weight of greater than 100,000 daltons.