O-succinyl homoserine transferase mutant and method for producing O-succinyl homoserine using same
The present disclosure relates to an O-succinyl homoserine transferase mutant, a polynucleotide encoding the same, a microorganism including the mutant, and a method of producing O-succinyl homoserine using the microorganism.
1. An isolated variant polypeptide having O-succinyl homoserine transferase activity, which comprises a substitution of an amino acid at position 313 in the amino acid sequence of SEQ ID NO: 1 with arginine and a substitution of an amino acid at position 176 in the amino acid sequence of SEQ ID NO: 1 with an amino acid other than glutamine and wherein the variant has at least 90% sequence identity to SEQ ID NO:1.
2. The isolated polypeptide of claim 1 , wherein the amino acid at position 176 is substituted with asparagine, tryptophan, histidine, or glycine.
3. The isolated polypeptide of claim 1 , wherein the polypeptide comprises at least one amino acid sequence selected from the group consisting of amino acid sequences of SEQ ID NOs: 63, 75, 95, and 97.
4. An isolated polynucleotide encoding the polypeptide having the O-succinyl homoserine transferase activity of claim 1 .
5. The isolated polynucleotide of claim 4 , wherein the polynucleotide comprises at least one nucleic acid sequence selected from the group consisting of nucleic acid sequences of SEQ ID NOs: 64, 76, 96, and 98.
6. A microorganism of the genus Corynebacterium producing O-succinyl homoserine, wherein the microorganism comprises the polypeptide having the O-succinyl homoserine transferase activity of claim 1 or a mutant polypeptide thereof or wherein, in the microorganism, the polypeptide having the O-succinyl homoserine transferase activity of claim 1 or a mutant polypeptide thereof is overexpressed.
7. The microorganism of claim 6 , wherein the microorganism of the genus Corynebacterium has further enhanced aspartokinase activity compared to non-mutated microorganisms.
8. The microorganism of claim 6 , wherein the microorganism of the genus Corynebacterium is Corynebacterium glutamicum.
9. The microorganism of claim 6 , wherein activity of at least one enzyme selected from the group consisting of cystathionine synthase, O-acetyl homoserine (thiol)-lyase, and homoserine kinase is inactivated in the microorganism of the genus Corynebacterium.
10. The microorganism of claim 6 , wherein the microorganism of the genus Corynebacterium has further enhanced aspartokinase activity compared to non-mutated microorganisms and activity of at least one enzyme selected from the group consisting of cystathionine synthase, O-acetyl homoserine (thiol)-lyase, and homoserine kinase is inactivated.
11. A method of producing O-succinyl homoserine, the method comprising:
culturing a microorganism of the genus Corynebacterium producing O-succinyl homoserine according to claim 6 in a culture medium; and
separating or recovering O-succinyl homoserine from the microorganism cultured in the culturing step or the culture medium.
12. A method of producing L-methionine, the method comprising:
(a) culturing a microorganism of the genus Corynebacterium producing O-succinyl homoserine according to claim 6 in a culture medium; and
(b) reacting the O-succinyl homoserine with a sulfide.
13. The method of claim 12 , further comprising separating or recovering O-succinyl homoserine from the cultured microorganism or the culture medium in step (a).
14. The method of claim 12 , further comprising separating or recovering L-methionine produced by the reaction between O-succinyl homoserine and the sulfide in step (b).