Combined organ and hematopoietic cells for transplantation tolerance of grafts
Methods and compositions are provided for combined transplantation of a solid organ and hematopoietic cells to a recipient, where tolerance to the graft is established through development of a persistent mixed chimerism. An individual with persistent mixed chimerism, usually for a period of at least six months, is able to withdraw from the use of immunosuppressive drugs after a period of time sufficient to establish tolerance.
1. A method of manufacturing a cellular product for establishing mixed chimerism in a solid organ transplant recipient, the method comprising:
obtaining one or more samples comprising CD34+ cells and CD3+ cells;
processing the one or more samples to substantially remove platelets and red blood cells; and
further processing the one or more samples to produce a cellular product comprising greater than 5×105 CD34+ cells/kg recipient weight, and greater than 1×105 CD3+ cells/kg recipient weight, wherein the cellular product is substantially devoid of platelets and red blood cells.
2. The method of claim 1 , wherein the one or more samples are further processed such that the cellular product comprises at least about 4×106 CD34+ cells/kg recipient weight.
3. The method of claim 1 , wherein the one or more samples are further processed such that the cellular product comprises at least about 1×106 CD3+ cells/kg recipient weight.
4. The method of claim 1 , wherein the one or more samples are further processed such that the cellular product comprises at least about 4×106 CD34+ cells/kg recipient weight and about 1×106 CD3+ cells/kg recipient weight.
5. The method of claim 1 , wherein the one or more samples are further processed such that the cellular product comprises at least about 4×106 CD34+ cells/kg recipient weight and at least about 1×106 CD3+ cells/kg recipient weight.
6. The method of claim 1 , wherein the cellular product is produced such that the CD34+ cells and the CD3+ cells are provided in separate containers.
7. The method of claim 1 , wherein the cellular product is produced such that the CD34+ cells and the CD3+ cells are provided as a mixture in a common container.
8. The method of claim 1 , wherein the CD34+ cells and the CD3+ cells are HLA matched to an HLA type of the solid organ transplant recipient.
9. The method of claim 1 , wherein the CD34+ cells and the CD3+ cells are HLA mismatched to an HLA type of the solid organ transplant recipient.
10. The method of claim 1 , wherein CD34+ cells and the CD3+ cells are from a single apheresis product.