Use of car and bite technology coupled with an SCFV from an antibody against human thymidine kinase 1 to specifically target tumors
A nucleic acid encoding a chimeric antigen receptor (CAR) comprising a single-chain variable fragment (scFv) operatively linked to a signaling domain that polarizes a macrophage to an M1 macrophage; wherein the nucleic acid is operatively linked to a macrophage specific promoter; and wherein the scFv is specific for a human antigen. Monocytes or macrophages comprising such a nucleic acid.
1. A nucleic acid encoding a chimeric antigen receptor (CAR) comprising a single-chain variable fragment (scFv) operatively linked to a signaling domain that polarizes a macrophage to an M1 macrophage;
wherein the nucleic acid is operatively linked to a macrophage specific promoter; and
wherein the scFv is specific for a human antigen.
2. The nucleic acid of claim 1 , wherein the signaling domain that polarizes a macrophage to an M1 macrophage is a human signaling domain that polarizes a macrophage to an M1 macrophage.
3. A monocyte or a macrophage comprising the nucleic acid of claim 1 .
4. The cell of claim 3 , wherein the monocyte is a human monocyte or wherein the macrophage is a human macrophage.
5. The nucleic acid of claim 1 , wherein the signaling domain is a TLR4 signaling domain.
6. A nucleic acid encoding a chimeric antigen receptor (CAR) comprising a single-chain variable fragment (scFv) operatively linked to a signaling domain that polarizes a macrophage to an M1 macrophage.