Method for extracorporeal removal of a pathogenic microbe, an inflammatory cell or an inflammatory protein from blood
The present invention relates to a method for extracorporeal removal of a pathogenic microbe, an inflammatory cell or an inflammatory protein from mammalian blood/use of a device comprising a carbohydrate immobilized on a solid substrate, said carbohydrate having a binding affinity for a pathogenic microbe, an inflammatory cell or an inflammatory protein, for extracorporeal removal of said pathogenic microbe, inflammatory cell or inflammatory protein from mammalian blood/use of a carbohydrate having a binding affinity for a pathogenic microbe, an inflammatory cell or an inflammatory protein, wherein said carbohydrate is immobilized on a solid substrate, in the preparation of a device for treatment of a condition caused or aggravated by said pathogenic microbe, inflammatory cell or inflammatory protein and a method for treatment of a mammalian subject suffering from a condition caused or aggravated by a pathogenic microbe, an inflammatory cell or an inflammatory protein.
1. A method for extracorporeal removal of an inflammatory cell from mammalian blood, comprising the steps:
a) providing a sample of mammalian blood,
b) bringing said sample into contact with a carbohydrate immobilized on a solid substrate, said carbohydrate having a binding affinity for an inflammatory cell, under conditions allowing binding of any inflammatory cells in said blood sample to the carbohydrate, wherein said immobilized carbohydrate is immobilized heparin having a terminal residue, wherein heparin immobilization consists of a single covalent link of said terminal residue to said solid substrate by covalent end-point attachment,
c) separating the sample from the solid substrate, such that said inflammatory cell is at least partially retained on the solid substrate, and
d) recovering said sample containing a reduced amount of said inflammatory cell.
2. The method according to claim 1 , wherein said inflammatory cell is selected from the group consisting of inflammatory lymphocytes and inflammatory macrophages.
3. The method according to claim 1 , wherein said mammalian blood is human blood.
4. The method according to claim 1 , wherein said carbohydrate further comprises one or more selected from the group consisting of, heparan sulfate, chondroitin sulfate, carbohydrates comprising sialic acid and carbohydrates comprising neuramic acid.
5. The method according to claim 1 , wherein said solid substrate comprises microparticles.
6. The method according to claim 1 , wherein said solid substrate comprises one or more hollow fibers.
7. The method according to claim 1 , wherein the material of said solid substrate is selected from the group consisting of glass, cellulose, cellulose acetate, chitin, chitosan, crosslinked dextran, crosslinked agarose, polypropylene, polyethylene, polysulfone, polyacrylonitrile, silicone, Teflon and polyurethanes.
8. The method according to claim 1 , wherein the carbohydrate immobilized on the solid substrate is disposed in a column.
9. The method according to claim 8 , wherein the column is a disposable column.