T cell balance gene expression, compositions of matters and methods of use thereof
This invention relates generally to compositions and methods for identifying the regulatory network that modulates, controls or otherwise influences T cell balance, for example, Th17 cell differentiation, maintenance and/or function, as well compositions and methods for exploiting the regulatory network that modulates, controls or otherwise influences T cell balance in a variety of therapeutic and/or diagnostic indications. This invention also relates generally to identifying and exploiting target genes and/or target gene products that modulate, control or otherwise influence T cell balance in a variety of therapeutic and/or diagnostic indications.
1. A method of modulating T cell balance, the method comprising:
contacting a Th17 cell or a population of T cells comprising Th17 cells with an exogenous T cell modulating agent in an amount sufficient to modify maintenance and/or function of the Th17 cell or population of T cells by altering balance between pathogenic and nonpathogenic Th17 cells as compared to maintenance and/or function of the Th17 cell or population of T cells in the absence of the T cell modulating agent, wherein the T cell modulating agent is a vector encoding for CD5L or a CRISPR-Cas9 system targeting the CD5L gene.
2. The method of claim 1 , wherein the T cells are naïve T cells, partially differentiated T cells, differentiated T cells, a combination of naïve T cells and partially differentiated T cells, a combination of naïve T cells and differentiated T cells, a combination of partially differentiated T cells and differentiated T cells, or a combination of naïve T cells, partially differentiated T cells and differentiated T cells.
3. The method of claim 1 , wherein the vector encoding for CD5L is a retroviral vector.
4. The method of claim 1 , wherein the Th17 cell or the population of T cells is contacted in vitro or ex vivo.