Controls for implementing multiplex analysis methods
The present invention relates to controls which may be used to secure the results of multiplex analysis methods. The present invention thus relates to solid supports comprising one or several controls and their use in multiplex analysis methods to detect several analytes potentially present in a sample.
1. A solid support appropriate for a multiplex analysis of at least one sample, said solid support comprising at least one compartment,
said compartment comprising a plurality of spots, each spot being deposited at a separate location within said at least one compartment,
said plurality of spots comprising two or more control spots deposited upon said solid support within said at least one compartment and at least two detection spots comprising a ligand specific for an analyte in a sample,
and said at least two control spots being selected from the group consisting of a spot for controlling the deposition of a sample, a spot for controlling the deposition of an analyte detection ligand and a spot for controlling the deposition of a reporter, wherein:
a) the spot for controlling the deposition of the sample comprises at least one control capture ligand specific for a control compound naturally present in the sample to be analyzed, said control capture ligand being an antibody, antigen, peptide, carbohydrate, lipid, or nucleic acid;
b) the spot for controlling the deposition of the detection ligand for the analyte comprises at least one additive capture ligand specific for an additive that is added to a sample and said additive is not naturally present in the sample or derived from any compound present in the sample, said at least one additive capture ligand being an antibody, antigen, peptide, carbohydrate, lipid, or nucleic acid;
c) the spot for controlling the deposition of the reporter comprises a direct marker, an indirect marker or a carrier molecule coupled to an indirect marker, wherein the reporter specifically interacts with the indirect marker or the indirect marker coupled to the carrier molecule, optionally, in presence of a substrate, to produce a detectable signal,
said direct marker being selected from a radioisotope, a fluorochrome, a lanthanide, a luminescent compound, a transition metal, and colored, fluorescent or luminescent nanoparticles,
said indirect marker being an enzyme, biotin, avidin, streptavidin, neutravidin, a hapten, an antigen or an antibody,
said carrier being a polypeptide, functionalized polymer, a copolymer, or an antibody, and
said reporter being an enzymatic substrate for an enzyme, an enzyme for a luminescent compound, and avidin, streptavidin, or neutravidin coupled to a direct or indirect marker for biotin, and
said ligands for analyte detection are antibodies, antigens, peptides, carbohydrates, lipids, or nucleic acids.
2. The solid support according to claim 1 , wherein one of the control spots is the spot for controlling the deposition of the sample.
3. The solid support according to claim 1 , wherein one of the control spots is the spot for controlling the deposition of the reporter.
4. The solid support according to claim 1 , wherein one of the control spots is the spot for controlling the deposition of the detection ligand of the analyte.
5. The solid support according to claim 1 , wherein the compartment comprises the spot for controlling the deposition of the sample and the spot for controlling the deposition of the detection ligand for the analyte.
6. The solid support according to claim 2 , wherein the compartment comprises the spot for controlling the deposition of the sample and the spot for controlling the deposition of the reporter.
7. The solid support according to claim 3 , wherein the compartment comprises the spot for controlling the deposition of the detection ligand for the analyte and the spot for controlling the deposition of the reporter.
8. The solid support according to claim 1 , said compartment comprising the spot for controlling the deposition of the sample; the spot for controlling the deposition of the detection ligand for the analyte; and the spot for controlling the deposition of the reporter.
9. The solid support according to claim 1 , wherein the at least one control capture ligand is an antibody specific to the control compound and the at least one additive capture ligand is an antibody specific to the additive.
10. The solid support according to claim 9 , wherein the at least one control capture ligand is an antibody specific to the control compound, the at least one additive capture ligand is an antibody specific to the additive, and the analyte detection ligand is an antibody specific for the analyte.
11. The solid support according to claim 1 , wherein the spots are cylindrical.
12. The solid support according to claim 1 , wherein the spots are discoid.