Feline calicivirus vaccine
The present invention provides new feline calicivirus vaccines, including multivalent vaccines. The present invention also provides methods of making and using the vaccines.
1. An immunogenic composition comprising an alphavirus RNA replicon particle that encodes a feline calicivirus (FCV) antigen.
2. The immunogenic composition of claim 1 , wherein the FCV antigen is a capsid protein or an antigenic fragment thereof.
3. The immunogenic composition of claim 2 , wherein the alphavirus RNA replicon particle is a Venezuelan Equine Encephalitis (VEE) alphavirus RNA replicon particle.
4. The immunogenic composition of claim 3 , wherein the capsid protein is selected from the group consisting of an FCV F9-Like capsid protein, an antigenic fragment of the FCV F9-Like capsid protein, a virulent systemic FCV (VS-FCV) capsid protein, and an antigenic fragment of the VS-FCV capsid protein.
5. The immunogenic composition of claim 4 , wherein the capsid protein is a VS-FCV capsid protein or an antigenic fragment thereof.
6. The immunogenic composition of claim 5 , that comprises an additional alphavirus RNA replicon particle that encodes an FCV F9-Like capsid protein or an antigenic fragment thereof.
7. The immunogenic composition of claim 5 , wherein the alphavirus RNA replicon particle also encodes an FCV F9-Like capsid protein, or an antigenic fragment of the FCV F9-Like capsid protein.
8. The immunogenic composition of claim 5 , wherein the VS-FCV capsid protein comprises an amino acid sequence comprising at least 95% identity with the amino acid sequence of SEQ ID NO: 2.
9. The immunogenic composition of claim 4 , wherein the capsid protein is a FCV F9-Like capsid protein or an antigenic fragment thereof.
10. The immunogenic composition of claim 9 , wherein the FCV F9-Like capsid protein comprises an amino acid sequence comprising at least 95% identity with the amino acid sequence of SEQ ID NO: 4.
11. A vaccine to aid in the prevention of disease due to FCV comprising the immunogenic composition of claim 4 , and a pharmaceutically acceptable carrier.
12. The vaccine composition of claim 11 , that further comprises at least one non-FCV antigen for eliciting protective immunity to a non-FCV feline pathogen.
13. The vaccine of claim 12 , wherein the non-FCV feline pathogen is selected from the group consisting of feline herpesvirus (FHV), feline leukemia virus (FeLV), feline pneumovirus (FPN), feline parvovirus (FPV), feline infectious peritonitis virus (FIPV), feline immunodeficiency virus, borna disease virus (BDV), feline influenza virus, feline panleukopenia virus (FPLV), feline coronavirus (FCoV), feline rhinotracheitis virus (FVR), Chlamydophila felis , and any combination thereof.
14. The vaccine of claim 12 , wherein the non-FCV antigen is an attenuated non-FCV antigen selected from the group consisting of feline herpesvirus (FHV), feline leukemia virus (FeLV), feline pneumovirus (FPN), feline parvovirus (FPV), feline infectious peritonitis virus (FIPV), feline immunodeficiency virus, borna disease virus (BDV), feline influenza virus, feline panleukopenia virus (FPLV), feline coronavirus (FCoV), feline rhinotracheitis virus (FVR), Chlamydophila felis , and any combination thereof.
15. The vaccine composition of claim 11 , that further comprises an alphavirus RNA replicon particle comprising a nucleotide sequence encoding at least one protein antigen or an antigenic fragment thereof that originates from a non- FCV antigen; wherein the protein antigen or an antigenic fragment thereof that originates from a non-FCV feline pathogen selected from the group consisting of feline herpesvirus (FHV), feline leukemia virus (FeLV), feline pneumovirus (FPN), feline parvovirus (FPV), feline infectious peritonitis virus (FIPV), feline immunodeficiency virus, borna disease virus (BDV), feline influenza virus, feline panleukopenia virus (FPLV), feline coronavirus (FCoV), feline rhinotracheitis virus (FVR), Chlamydophila felis , and any combination thereof.
16. The vaccine composition of claim 11 , that is a nonadjuvanted vaccine.
17. A method of immunizing a feline against a pathogenic FCV comprising administering to the feline an immunologically effective amount of the vaccine of claim 16 .
18. The immunogenic composition of claim 1 , that comprises an additional alphavirus RNA replicon particle that encodes a second FCV antigen, wherein the second FCV antigen originates from a different strain of FCV than the one from which the FCV antigen originates from.
19. The immunogenic composition of claim 18 , wherein the additional alphavirus RNA replicon particle is an VEE alphavirus RNA replicon particle.
20. A vaccine to aid in the prevention of disease due to FCV comprising a Venezuelan Equine Encephalitis (VEE) alphavirus RNA replicon particle that encodes a feline calicivirus (FCV) F9-Like capsid protein comprising an amino acid sequence that comprises at least 95% identity with the amino acid sequence of SEQ ID NO: 4 and a VS-FCV capsid protein comprising an amino acid sequence that comprises at least 95% identity with the amino acid sequence of SEQ ID NO: 2.