Sequence design for efficient assembly of nucleic acid structures
The present disclosure provides, in some aspects, methods and compositions for producing nucleic acid nanostructures having little to no kinetic barriers to self-assembly.
1. A nucleic acid nanostructure comprising:
(a) a deoxyribonucleic acid (DNA) strand having a length of at least 100 nucleotides and having a nucleotide sequence that comprises less than 10% of one of C, G, T or A nucleotides; and
(b) 10-2000 oligonucleotides, wherein each oligonucleotide is at least partially complementary to and at least partially bound to the DNA strand.
2. The nucleic acid nanostructure of claim 1 , wherein the nucleotide sequence comprises greater than 90% of A, T and C nucleotides and less than 10% of G nucleotides.
3. The nucleic acid nanostructure of claim 1 , wherein the nucleotide sequence comprises greater than 90% of A, T and G nucleotides and less than 10% of C nucleotides.
4. The nucleic acid nanostructure of claim 1 , wherein the nucleotide sequence comprises less than 5% of one of C, G, T or A nucleotides.
5. The nucleic acid nanostructure of claim 1 , wherein the nucleotide sequence comprises 1-5% of one of C, G, T or A nucleotides.
6. The nucleic acid nanostructure of claim 1 , wherein the nucleotide sequence comprises nucleotide subsequences, each of which is present only once in the DNA strand.
7. The nucleic acid nanostructure of claim 6 , wherein the nucleotide subsequences have a length of 5 to 10 nucleotides.
8. The nucleic acid nanostructure of claim 1 , wherein the DNA strand has a length of at least 500 nucleotides.
9. The nucleic acid nanostructure of claim 1 , wherein the DNA strand has a length of at least 1000 nucleotides.
10. The nucleic acid nanostructure of claim 9 , wherein the DNA strand has a length of 100-100,000 nucleotides.
11. The nucleic acid nanostructure of claim 1 , wherein the nucleic acid nanostructure is three-dimensional.
12. A nucleic acid nanostructure of comprising:
(a) a deoxyribonucleic acid (DNA) strand having a length of at least 100 nucleotides and having a nucleotide sequence that comprises less than 10% of one of C, G, T or A nucleotides; and
(b) a plurality of oligonucleotides, wherein each oligonucleotide is at least partially complementary to and at least partially bound to the DNA strand, wherein the nanostructure is a three-dimensional DNA origami structure and has a diameter of 10-500 nm.
13. A kit comprising
(a) a deoxyribonucleic acid (DNA) strand having a length of at least 100 nucleotides and having a nucleotide sequence that comprises less than 10% of one of C, G, T or A nucleotides; and
(b) 10-2000 oligonucleotides, wherein each oligonucleotide is at least partially complementary to and at least partially bound to the DNA strand.
14. A method of producing a DNA origami nanostructure, comprising
incubating, in a reaction vessel,
(a) a deoxyribonucleic acid (DNA) strand having a length of at least 100 nucleotides and having a nucleotide sequence that comprises less than 10% of one of C, G, T or A nucleotides, and
(b) a plurality of oligonucleotides, wherein each oligonucleotide is at least partially complementary to the DNA strand,
under conditions that result in binding of the oligonucleotides to the DNA strand to form a DNA origami nanostructure.