Cartilage regenerative material
An object of the invention is to provide a cartilage regenerative material that is capable of regenerating bone and cartilage using cells. Provided is a cartilage regenerative material including a cell construct, which includes biocompatible polymer blocks and stem cells, in which a plurality of the biocompatible polymer blocks are disposed in gaps between a plurality of the stem cells.
1. A method for regenerating cartilage and bone, the method comprising transplanting a cell construct that includes biocompatible polymer blocks and stem cells to a patient in need of cartilage generation, in which the cell construct has a plurality of the biocompatible polymer blocks disposed in gaps between a plurality of the stem cells, wherein the cell construct and a biocompatible polymer film is transplanted, wherein the biocompatible polymer film is present between the transplantation site and the cell construct, and
wherein the bone and cartilage are generated at the same time.
2. The method of according to claim 1 , wherein the cell construct is transplanted to a diseased site of cartilage defect.
3. The method of according to claim 1 , wherein the stem cells are mesenchymal stem cells (MSC), amniotic cells, cord blood-derived cells, bone marrow-derived cells, or adipose-derived stem cells.
4. The method according to claim 1 , wherein the stem cells are mesenchymal stem cells (MSC).
5. The method according to claim 1 , wherein the biocompatible polymer film is a biodegradable polymer.
6. The method according to claim 1 , wherein the cell construct includes the biocompatible polymer blocks in an amount of from 0.0000001 μm to 1 μg per stem cell.
7. The method according to claim 1 , wherein the size of each of the biocompatible polymer blocks is from 10 μm to 300 μm.
8. The method according to claim 1 , wherein the cell construct has a thickness or diameter of from 100 μm to 1 cm.
9. The method according to claim 1 , wherein the biocompatible polymer blocks comprise a recombinant peptide or a chemically synthesized peptide.
10. The method according to claim 1 , wherein the biocompatible polymer blocks comprise a recombinant gelatin or a chemically synthesized gelatin.
11. The method according to claim 1 , wherein the biocompatible polymer blocks comprise a recombinant gelatin or a chemically synthesized gelatin represented by Formula 1,
A-[(Gly-X-Y) n ] m -B Formula 1:
in Formula 1, n units of X each independently represent any amino acid residue; n units of Y each independently represent any amino acid residue; m represents an integer from 2 to 10;
n represents an integer from 3 to 100; A represents an arbitrary amino acid residue or amino acid sequence; and B represents an arbitrary amino acid residue or amino acid sequence.
12. The method according to claim 1 , wherein the biocompatible polymer blocks comprise any of the following:
a peptide comprising the amino acid sequence set forth in SEQ ID NO:1;
a peptide having biocompatibility and comprising an amino acid sequence obtained by modifying the amino acid sequence set forth in SEQ ID NO:1 by deletion, substitution or addition of one or several amino acid residues; or
a peptide having biocompatibility and comprising an amino acid sequence having at least 80% sequence identity with the amino acid sequence set forth in SEQ ID NO:1.
13. The method according to claim 1 , wherein biocompatible polymers in the biocompatible polymer blocks are crosslinked by heat, ultraviolet radiation, or an enzyme.
14. The method according to claim 1 , wherein the biocompatible polymer blocks are in the form of granules obtainable by pulverizing a porous body of a biocompatible polymer.
15. The method according to claim 1 wherein the cell construct and a biocompatible polymer film is transplanted, and the biocompatible polymer film is a film for isolating a portion or the entirety of the transplant face of the cell construct from the site of transplantation.