Dry-down processes for dye-conjugated reagents
The method involves drying down dye-conjugated reagents in separate locations in a reaction vessel so that the dyes don't non-specifically interact with each other during drying. This invention thus improves multiplex binding assays by eliminating erroneous results caused by dyes' being non-specifically attached to each other when dried down together.
1. A reaction vessel comprising a body comprising:
a first dry reaction spot disposed on a first substrate, the first dry reaction spot comprising a first binding partner conjugated to a first dye, wherein the first dye comprises a polymer dye; and
a second dry reactant spot disposed on a second substrate, the second dry reactant spot comprising a second binding partner conjugated to a second dye, wherein the first binding partner has a different affinity for a target analyte than the second binding partner;
wherein the first substrate and the second substrate are bonded to an inside surface of the body of the reaction vessel, the first substrate and the second substrate independently comprising plastic, nitrocellulose, cellulose acetate, glass, a membrane, a bead, or a combination thereof, and
wherein the reaction vessel is configured to receive a liquid sample, such that the liquid sample contacts the first binding partner and the second binding partner to form a liquid reaction mixture comprising the first binding partner and the second binding partner in the reaction vessel.
2. The reaction vessel of claim 1 , wherein at least one of the first substrate and the second substrate comprises a membrane.
3. The reaction vessel of claim 1 , wherein the second substrate is layered above the first substrate.
4. The reaction vessel of claim 1 , wherein the bonding of the first substrate and second substrate to the inside surface of the body of the reaction vessel prevents the first substrate and the second substrate from escaping the reaction vessel during pipetting of liquid into or out of the reaction vessel.
5. The reaction vessel of claim 1 , wherein at least one of the first substrate and the second substrate comprises a bead.
6. The reaction vessel of claim 1 , wherein at least one of the first dry reaction spot and the second dry reaction spot is in the form of a film.
7. The reaction vessel of claim 1 , wherein the second dye comprises a polymer dye.
8. A method of preparing two or more dry reactant spots, the method comprising:
dispensing a first reactant in liquid phase onto a first substrate, the first reactant including a binding partner conjugated to a first dye, wherein the first dye comprises a polymer dye;
dispensing a second reactant in liquid phase onto a second substrate, the second reactant including a second binding partner conjugated to a second dye, wherein the first binding partner has a different affinity for a target analyte than the second binding partner;
drying the first reactant and the second reactant in liquid phase to form a first dry reactant spot on the first substrate and a second dry reactant spot on the second substrate; and
bonding the first substrate and the second substrate to an inside surface of a reaction vessel.
9. The method of claim 8 , wherein at least one of the first substrate and the second substrate comprises a membrane.
10. The method of claim 8 , wherein at least one of the first substrate and the second substrate comprises a bead.
11. The method of claim 8 , wherein the bonding of the first substrate and/or second substrate to the inside surface of the body of the reaction vessel prevents the bonded substrate from escaping the reaction vessel during pipetting of liquid into or out of the reaction vessel.
12. The method of claim 8 , wherein the second dye comprises a polymer dye.
13. A method of preparing two or more dry reaction spots, the method comprising:
drying a first reactant in liquid phase onto a first substrate to form a first dry reactant spot, the first reactant including a first binding partner conjugated to a first dye, wherein the first dye comprises a polymer dye;
drying a second reactant in liquid phase onto a second substrate to form a second dry reactant spot, the second reactant including a second binding partner conjugated to a second dye, wherein the first binding partner has a different affinity for a target analyte than the second binding partner; and
bonding the first substrate and the second substrate to an inside surface of a reaction vessel;
wherein the reaction vessel is configured to receive a liquid sample, such that the liquid sample contacts the first dry reactant spot and the second dry reactant spot and dissolves the first binding partner and the second binding partner to form a liquid reaction mixture comprising the first binding partner and the second binding partner in the reaction vessel.
14. The method of claim 13 , wherein at least one of the first substrate and the second substrate is a membrane.
15. The method of claim 13 , wherein at least one of the first substrate and the second substrate is a bead.
16. The method of claim 13 , wherein at least one of the first dry reactant spot and the second dry reactant spot is in the form of a film.
17. The method of claim 13 , wherein the bonding of the first substrate and/or second substrate to the inside surface of the body of the reaction vessel prevents the bonded substrate from escaping the reaction vessel during pipetting of liquid into or out of the reaction vessel.