Peptides selective for Bcl-2 family proteins
Provided herein are peptides selective for combinations of Mcl-1/Bfl- 1 /Bcl-xL. Also provided are compositions containing these polypeptides and methods of using such peptides in the treatment of cancer that include administering to a subject one of the polypeptides.
1. A compound comprising peptide comprising an amino acid sequence selected from the group consisting of:
(SEQ ID NO: 85)
RSELEVVQELVRIGDIVVAYFER;
(SEQ ID NO: 86)
RSQYEVIQELIRIGDIVLAYFER;
(SEQ ID NO: 87)
DVVLSVAETLRELADRLYEEINT;
(SEQ ID NO: 88)
QRVVHIAAGLRRTGDQLEAYG;
(SEQ ID NO: 89)
RRVVQIAAGLRRAGDQLEKYG;
(SEQ ID NO: 90)
SYVDKIADVMREVAEKINSDLT;
(SEQ ID NO: 91)
SLLEKLAEYLRQMADEINKKYVK;
(SEQ ID NO: 92)
QRIIWIAAELRRAADELDKQIER;
(SEQ ID NO: 93)
QRIIWIAAELRRAADQLDAQIER;
(SEQ ID NO: 94)
RWIDQIAQFLRRIGDHIEKYIER;
(SEQ ID NO: 95)
RRVDEIAQILRRIGDNVTTYIER;
(SEQ ID NO: 96)
QWLRWVIAELIRIADEFHAQYER;
and
(SEQ ID NO: 97)
QWLRDVVAELARIADEFHAQYER.
2. The compound of claim 1 , comprising a peptide comprising an amino acid sequence selected from the group consisting of:
(SEQ ID NO: 85)
RSELEVVQELVRIGDIVVAYFER;
(SEQ ID NO: 86)
RSQYEVIQELIRIGDIVLAYFER;
and
(SEQ ID NO: 87)
DVVLSVAETLRELADRLYEEINT.
3. The compound of claim 1 , comprising a peptide comprising an amino acid sequence selected from the group consisting of:
(SEQ ID NO: 88)
QRVVHIAAGLRRTGDQLEAYG;
(SEQ ID NO: 89)
RRVVQIAAGLRRAGDQLEKYG;
(SEQ ID NO: 90)
SYVDKIADVMREVAEKINSDLT;
and
(SEQ ID NO: 91)
SLLEKLAEYLRQMADEINKKYVK.
4. The compound of claim 1 , comprising a peptide comprising an amino acid sequence selected from the group consisting of:
(SEQ ID NO: 92)
QRIIWIAAELRRAADELDKQIER;
and
(SEQ ID NO: 93)
QRIIWIAAELRRAADQLDAQIER.
5. The compound of claim 1 , comprising a peptide comprising an amino acid sequence selected from the group consisting of:
(SEQ ID NO: 94)
RWIDQIAQFLRRIGDHIEKYIER;
and
(SEQ ID NO: 95)
RRVDEIAQILRRIGDNVTTYIER.
6. The compound of claim 1 , comprising a peptide comprising an amino acid sequence selected from the group consisting of:
(SEQ ID NO: 96)
QWLRWVIAELIRIADEFHAQYER;
and
(SEQ ID NO: 97)
QWLRDVVAELARIADEFHAQYER.
7. A pharmaceutical composition comprising the compound of claim 1 .
8. A method of treating cancer comprising the administering of the compound of claim 1 .
9. A method for detecting a BH3 binding domain peptide in a cell, the method comprising:
a) providing cell;
b) contacting the cell with a compound claim 1 ; and
d) determining whether the compound binds to the cell.