Microfluidic device with integrated waveguides for analyte identification and method of operation thereof
A microfluidic device and a method of detecting the presence of an analyte in a fluid. In one embodiment, the microfluidic device includes: (1) a substrate, (2) a waveguide supported by the substrate and configured to receive light and (3) a microfluidic channel contacting the waveguide and configured to convey a fluid, a characteristic of the light changing under influence of an analyte in the fluid.
1. A microfluidic device, comprising:
a substrate;
a first waveguide supported by said substrate and configured to convey a first light stream from a first light source;
a second waveguide supported by said substrate and configured to convey a second light stream from a second light source; and
a microfluidic channel structure comprising a first channel, a second channel, and a merged channel, wherein each of said first channel, said second channel, and said merged channel are formed by said first and second waveguides and a plurality of partitioning features that cooperate with each other to form walls for said first channel, said second channel, and said merged channel;
wherein;
said merged channel contacts said first waveguide and is configured to convey a fluid comprising a first analyte and second analyte, wherein a first characteristic of said first light stream in said first waveguide is configured to change under an influence of the first analyte in said fluid in said merged channel; and
said second channel contacts said second waveguide and is configured to convey a fluid comprising a first analyte and a second analyte, wherein a second characteristic of said second light stream in said second waveguide is configured to change under an influence of the second analyte in said fluid in said second channel.
2. The microfluidic device as recited in claim 1 wherein said first and second analytes are selected from the group consisting of:
a bacterium,
a peptide,
a lipid,
a protein,
a virus,
a cell, and
a fungus.
3. The microfluidic device as recited in claim 1 wherein said first and second light streams are infrared light.
4. The microfluidic device as recited in claim 1 wherein said first and second characteristics are absorption spectrums of said first and second light streams.
5. The microfluidic device as recited in claim 1 wherein said influence of said first analyte and said influence of said second analyte are attenuated total reflection.
6. The microfluidic device as recited in claim 1 wherein said first and second waveguides and said plurality of partitioning features are formed from said substrate.
7. The microfluidic device as recited in claim 1 wherein a sealing material seals said first channel, said second channel, and said merged channel.
8. The microfluidic device as recited in claim 1 wherein each of said first and second waveguides comprise a cladding layer.
9. The microfluidic device as recited in claim 1 wherein a film coats walls of said microfluidic channel structure, including portions of said first and second waveguides, said film configured to increase said influence of said first analyte and said influence of said second analyte.
10. The microfluidic device as recited in claim 1 wherein a metal coats said first and second waveguides, said metal configured to increase surface-enhanced infrared absorption.
11. The microfluidic device as recited in claim 1 further comprising a first shutter configured to perform one of:
intermittently blocking said first light stream from entering said first waveguide, and
intermittently blocking said first light stream exiting said first waveguide.
12. A microfluidic device, comprising:
a substrate;
a first waveguide supported by said substrate and configured to convey a first light stream from a first end face to a second end face thereof;
a first light source configured to provide said first light stream to said first end face of said first waveguide;
a second waveguide supported by said substrate and configured to convey a second light stream from a first end face to a second end face thereof;
a second light source configured to provide said second light stream to said first end face of said second waveguide;
a first light detector configured to receive light from said second end face of said first waveguide and produce a first signal based thereon;
a second light detector configured to receive light from said second end face of said second waveguide and produce a second signal based thereon; and
a microfluidic channel structure comprising a first channel, a second channel, and a merged channel, wherein each of said first channel, said second channel, and said merged channel are formed by said first and second waveguides and a plurality of partitioning features that cooperate with each other to form walls for said first channel, said second channel, and said merged channel;
wherein;
said merged channel contacts said first waveguide and is configured to convey a fluid comprising a first analyte and a second analyte, wherein a first characteristic of said first light stream in said first waveguide is configured to change under an influence of the first analyte in said fluid in said merged channel, said first characteristic being evident in said first signal; and
said second channel contacts said second waveguide and is configured to convey a fluid comprising a first analyte and a second analyte, wherein a second characteristic of said second light stream in said second waveguide is configured to change under an influence of the second analyte in said fluid in said second channel said second characteristic being evident in said second signal.
13. The microfluidic device as recited in claim 12 wherein said first and second analytes are selected from the group consisting of:
a bacterium,
a peptide,
a lipid,
a protein,
a virus,
a cell, and
a fungus.
14. The microfluidic device as recited in claim 12 wherein said influence of said first analyte and said influence of said second analyte are attenuated total reflection.
15. The microfluidic device as recited in claim 12 wherein a film coats walls of said microfluidic channel structure, including portions of said first and second waveguides, said film configured to increase said influence of said first analyte and said influence of said second analyte.
16. The microfluidic device as recited in claim 12 wherein a metal coats said first and second waveguides, said metal configured to increase surface-enhanced infrared absorption.
17. The microfluidic device as recited in claim 12 further comprising at least one of:
a first shutter located between said first light source and said first end face and configured intermittently to block said first light stream from entering said first waveguide, and
a second shutter located between said second end face and said first light detector and configured intermittently to block said first light stream from entering said first light detector.