Use of genomic signatures to predict responsiveness of patients with prostate cancer to post-operative radiation therapy
Methods, compositions, and kits for identifying individuals who will be responsive to post-operative radiation therapy for treatment of prostate cancer are disclosed. In particular, the invention relates to a genomic signature based on expression levels of DNA Damage Repair genes that can be used to identify individuals likely to benefit from post-operative radiation therapy after a prostatectomy.
1. A method for treating a subject having prostate cancer, comprising:
a) obtaining a post-operative radiation therapy outcome score (PORTOS) that has been calculated using a level of expression of a plurality of genes in the biological sample, wherein said plurality of genes comprises DRAM1, KRT14, PTPN22, ZMAT3, ARHGAP15, IL1B, ANLN, RPS27A, MUM1, TOP2A, GNG11, CDKN3, HCLS1, DTL, IL7R, UBA7, NEK1, CDKN2AIP, APEX2, KIF23, SULF2, PLK2, EME1, and BIN2;
b) determining whether or not the subject is likely to benefit from post-operative radiation therapy based on the PORTOS score, wherein a PORTOS greater than 0 indicates that the subject will benefit from the post-operative radiation therapy and a PORTOS less than or equal to 0 indicates that the subject will not benefit from the post-operative radiation therapy; and
c) if the PORTOS indicates that the subject will benefit from the radiation therapy, then administering the post-operative radiation therapy to the subject, and if the PORTOS does not indicate that the subject will benefit from the post-operative radiation therapy, then administering a cancer treatment other than the post-operative radiation therapy to the subject.
2. The method of claim 1 , wherein the subject has previously undergone a radical prostatectomy.
3. The method of claim 1 , wherein the prostate cancer has not metastasized.
4. The method of claim 1 , wherein the biological sample is a biopsy.
5. The method of claim 1 , wherein the biological sample is a tumor sample.
6. The method of claim 1 , wherein the subject is a human being.
7. The method of claim 1 , wherein the level of expression has been measured by performing microarray analysis, polymerase chain reaction (PCR), reverse transcriptase polymerase chain reaction (RT-PCR), a Northern blot, or serial analysis of gene expression (SAGE).
8. The method of claim 1 , wherein said administering post-operative radiation therapy to the subject, or administering a cancer treatment other than the post-operative radiation therapy to the subject further comprises performing chemotherapy, immunotherapy, hormonal therapy, biologic therapy, or any combination thereof.
9. The method of claim 1 , wherein the PORTOS indicates that the subject will benefit from the radiation therapy, and administering the post-operative radiation therapy to the subject.