Methods and compositions for the introduction and regulated expression of genes in plants
Compositions and methods are provided for the introduction and the regulated expression of genes in plants. Compositions include promoter constructs that provide a level of activity useful for the regulated expression of site-specific recombinases, while avoiding premature excision. Further provided are isolated polynucleotides encoding novel babyboom polypeptides, expression cassettes, and plants comprising the same. Methods for the introduction of genes into plants are provided, including methods for plastid transformation and methods for the transformation of tissues from mature seeds and leaves.
1. A method for introducing a polynucleotide of interest into a maize leaf tissue explant, said method comprising:
a) excising a leaf segment from a leaf above the first leaf base node;
b) dissecting said leaf segment into a leaf tissue explant;
c) introducing into said leaf tissue explant: i) a heterologous polynucleotide encoding a babyboom cell proliferation factor and a heterologous polynucleotide encoding a Wuschel cell proliferation factor; and expressing said heterologous polynucleotide encoding said babyboom cell proliferation factor and said heterologous polynucleotide encoding said Wuschel cell proliferation factor; and ii) a polynucleotide of interest; and
d) regenerating a monocot plant comprising the polynucleotide of interest.
2. The method of claim 1 , further comprising excising said heterologous polynucleotide encoding said babyboom cell proliferation factor and said heterologous polynucleotide encoding said Wuschel cell proliferation factor.