Apparatus and methods for concentrating platelet-rich plasma
Apparatus and methods for concentrating platelet-rich plasma is described herein. One variation may generally comprise a tube having a length and defining a channel within and one or more ports located at a proximal end of the tube and in fluid communication with the channel. A plunger may slidably translatable within the channel while forming a seal against an inner surface of the channel and a float may have a pre-selected density and defining a concave interface surface, wherein the float is slidably contained within the channel such that the concave interface surface is in apposition to the one or more ports.
1. A separation apparatus, comprising
a tube having a length and defining a channel within;
one or more ports located at a proximal end of the tube and in fluid communication with the channel;
a plunger slidably translatable within the channel while forming a seal against an inner surface of the channel;
a float having a pre-selected density and defining an interface surface having a concave shape, wherein the float is slidably contained within the channel such that the interface surface is in apposition to the one or more ports and defines an angle of 2 to 60 degrees relative to a transverse plane of the float such that the interface surface is configured to preferentially pool blood components along the interface surface.
2. The apparatus of claim 1 further comprising a vent or opening defined along the tube distal to the plunger.
3. The apparatus of claim 1 wherein the one or more ports comprise a blood introduction port, a PPP withdrawal port, and a buffy coat withdrawal port.
4. The apparatus of claim 1 wherein the plunger is configured to form a seal against a bottom surface of the tube.
5. The apparatus of claim 1 wherein the float has a density between a layer of RBC and a layer of PPP when the tube is centrifuged.
6. The apparatus of claim 1 wherein the interface surface comprises a concave interface surface which is configured to retain a buffy coat layer when the tube is centrifuged.
7. The apparatus of claim 1 further comprising a pull rod attachable to the plunger.
8. The apparatus of claim 1 wherein the float comprises a portion extending from the float for contact against an upper inner surface of the tube.
9. The apparatus of claim 1 wherein the plunger defines an opening sized to receive a projection extending from the float.
10. The apparatus of claim 1 wherein the interface surface is angled such that the blood components are preferentially pooled between the inner surface of the channel and the interface surface.
11. A method of separating components from blood, comprising:
displacing a float and a plunger from a first position within a tube to a second position by introducing a volume of blood into the tube, the tube having a length and defining a channel within and the plunger being slidably translatable within the channel while forming a seal against an inner surface of the channel;
applying a centrifugal force to the volume of blood contained within the tube such that the blood forms at least a first fractional layer and a second fractional layer and the float positions itself between the first and second fractional layers, wherein the float has a pre-selected density and defines an interface surface having a concave shape and where the interface surface defines an angle of 2 to 60 degrees relative to a transverse plane of the float such that the interface surface is configured to preferentially pool blood components along the interface surface; and
withdrawing at least the first fractional layer from the tube via at least one port defined at a proximal end of the tube and in fluid communication with the channel such that the float and plunger are moved from the second position back towards the first position, wherein the float is slidably contained within the channel such that the interface surface is in apposition to the at least one port.
12. The method of claim 11 further comprising venting a volume of air into or from the tube while the plunger is translated within the tube.
13. The method of claim 11 further comprising introducing an anticoagulant into the tube prior to introducing the volume of blood into the tube.
14. The method of claim 11 wherein withdrawing at least the first fractional layer comprises withdrawing a PPP layer.
15. The method of claim 14 further comprising withdrawing a third fractional layer comprising a buffy coat from the concave interface surface.
16. The method of claim 11 wherein the second fractional layer comprises a RBC layer.
17. The method of claim 11 wherein displacing a float and a plunger comprises forming a vacuum within the tube.
18. The method of claim 11 wherein withdrawing at least the first fractional layer comprises withdrawing from the interface surface of the float.