Method for preparing of nerve conduit using bio-printing technology and the nerve conduit prepared by the same

1. A method for preparing a nerve conduit, comprising

printing bio-ink on one side or both sides of a porous polymer membrane using a three-dimensional bio-printer (3D bio-printer); and

preparing a nerve conduit by wrapping the porous polymer membrane in which the bio-ink is printed in a conduit form;

wherein the bio-ink comprises a collagen hydrogel; wherein the porous polymer membrane comprises poly(L-lactide-co-caprolactone); and wherein the bio-ink is printed in a plurality of lines in one direction.

2. The method for preparing a nerve conduit according to claim 1 ,

wherein the porous polymer membrane is wrapped into a tubular form, a winding form, or a fiber bundle form.

3. The method for preparing a nerve conduit according to claim 1 ,

A method for preparing a nerve conduit, comprising printing bio-ink on one side or both sides of a plurality of porous polymer membranes using a 3D bio-printer; stacking the plurality of porous polymer membranes; and wrapping the stacked plurality of porous polymer membranes in a multi-pipe form; wherein the bio-ink comprises a collagen hydrogel; and wherein the porous polymer membrane comprises poly(L-lactide-co-caprolactone).

4. The method for preparing a nerve conduit according to claim 1 ,

wherein the porous polymer membrane is in a sheet form obtained by electrospinning.

5. The method for preparing a nerve conduit according to claim 1 ,

wherein the collagen hydrogel further comprises a decellularized extracellular matrix (dECM) powder in an amount of 5% by weight or less based on the total weight of the collagen hydrogel.

6. The method for preparing a nerve conduit according to claim 1 , wherein the bio-ink further comprises a neuroregenerative factor;

wherein the neuroregenerative factor is at least one selected from the group consisting of fibroblast growth factor (FGF), bone morphogenetic proteins (BMPs), granulocyte colony-stimulating factor (G-CSF), granulocyte-macrophage colony-stimulating factor (GM-CSF), epidermal growth factor (EGF), growth differentiation factor (GDF), insulin-like growth factor (IGF), interleukin (IL), migration-stimulating factor (MSF), macrophage-stimulating protein (MSP), neurotrophin (NGF), platelet-derived growth factor (PDGF), transforming growth factor (TGF), vascular endothelial growth factor (VEGF) and neurotrophic factor family (NTF).

7. The method for preparing a nerve conduit according to claim 1 , wherein the bio-ink further comprises a cell;

wherein the cell is at least one selected from the group consisting of neurons and stem cells.

8. The method for preparing a nerve conduit according to claim 1 ,

wherein the bio-ink comprises a first bio-ink comprising a collagen hydrogel and a neurogenerative factor; and second bio-ink comprising a collagen hydrogel and a neuron.

9. The method for preparing a nerve conduit according to claim 1 ,

wherein the first bio-ink comprises a neuron, a neuroregenerative factor, and a collagen hydrogel; and the second bio-ink comprising a stem cell, a neuroregenerative factor, and a collagen hydrogel, wherein the neuroregenerative factor in the first bio-ink and the second bio-ink are different.

10. The method for preparing a nerve conduit according to claim 9 ,

wherein the three-dimensional bio-printer comprises at least two printing nozzles, and

the first bio-ink and the second bio-ink are printed by separate printing nozzles, respectively.

11. The method for preparing a nerve conduit according to claim 1 ,

further comprising applying an adhesive for biological tissue on the surface of the nerve conduit.

12. A nerve conduit prepared by the method of claim 1 .

13. The nerve conduit according to claim 12 ,

wherein the nerve conduit is for central nerve or peripheral nerve regeneration.