IP Library Granted Patent US 11,633,741
Granted Patent B2
US 11,633,741 · App. 16/794,937 · Granted Apr 25, 2023

Slide chamber

Inventors: Eiad Kabaha (Cologne, DE); Ralf-Peter Peters (Bergisch Gladbach, DE); Jan Keseberg (Altendorn, DE); Andreas Bosio (Cologne, DE)
Assignee: Miltenyi Biotec B.V. & Co. KG
B01L9/52B01L2200/0689B01L2200/087B01L2300/046B01L2300/12
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Quick Facts
Patent No.
US 11,633,741
App. No.
16/794,937
Granted
Apr 25, 2023
Kind
B2
Abstract

The invention is directed to a slide chamber comprising a top member ( 10 ), a fluidic seal ( 20 ), a transparent closure member ( 30 ) and base member ( 40 ) wherein the top member ( 10 ) is provided with at least one examination chamber ( 11 ) and a plurality of openings ( 12 ) positioned at two sides of the cover member outside of the examination chamber ( 11 ); and the base member ( 40 ) is provided with interconnecting means ( 41 ) complementary to the openings ( 12 ) of the top member characterized in that the interconnecting means ( 41 ) of the base member ( 40 ) and the openings ( 12 ) of the top member ( 10 ) are configured to mechanically interlock with each other by lateral movement thereby pressing the top member ( 10 ) against the transparent closure ( 30 ) member in a water-tight manner.

Claims (20)

1. A slide chamber comprising a top member ( 10 ), a fluidic seal ( 20 ), a transparent closure member ( 30 ) and base member ( 40 ) wherein the top member ( 10 ) is provided with at least one examination chamber ( 11 ) and a plurality of openings ( 12 ) positioned at two sides of the cover member outside of the examination chamber ( 11 ); and

the base member ( 40 ) is provided with interconnecting means ( 41 ) complementary to the openings ( 12 ) of the top member

characterized in that the interconnecting means ( 41 ) of the base member ( 40 ) and the openings ( 12 ) of the top member ( 10 ) are configured to mechanically interlock with each other by lateral movement the base member and the transparent closure member move laterally from an unsealed to a sealed, non-leaking position using the interconnecting means, thereby pressing the top member ( 10 ) against the transparent closure ( 30 ) member in the locked position wherein the interconnecting means ( 41 ) is configured as a bayonetted joint.

2. The slide chamber according to claim 1 , characterized in that the interconnecting means ( 41 ) and the openings ( 12 ) are configured to be mechanically disengaged from each other by lateral movement of the base member ( 40 ) against the top member ( 10 ).

3. The slide chamber according to claim 1 , characterized in that the top member ( 10 ) is provided with a recess for the fluidic seal ( 20 ).

4. The slide chamber according to claim 1 , characterized in that the top member ( 10 ) is provided with means for holding the transparent closure member ( 30 ).

5. The slide chamber according to claim 4 , characterized in that means for holding the transparent closure member ( 30 ) are configured to allow movement of the transparent closure member ( 30 ) against the top member ( 10 ).

6. The slide chamber according to claim 4 , characterized in that the means for holding the transparent closure member ( 30 ) are configured to allow movement of the transparent closure member ( 30 ) against the top member ( 10 ) before finally assembling the slide chamber.

7. The slide chamber according to claim 1 , characterized in that the top member ( 10 ) is provided with at least one grip ( 14 ).

8. The slide chamber according to claim 1 , characterized in that the interconnecting means ( 41 ) of the base member ( 40 ) and the openings ( 12 ) of the top member ( 10 ) are configured as bayonetted joint.

9. The slide chamber according to claim 1 , characterized in that the fluidic seal ( 20 ) has a Shore A hardness of 20-50.

10. The slide chamber according to claim 1 , characterized in that the base member ( 40 ) and the top member ( 10 ) is manufactured from PET, PS, PE, PP or COP.

11. The slide chamber according to claim 1 , characterized in that the transparent closure member ( 30 ) is a microscopic slide glass.

12. The slide chamber according to claim 1 , characterized in that the examination chambers are provided by walls attached to or emerging from the upper surface of the top member ( 10 ).

13. The slide chamber according to claim 1 , characterized in that the examination chambers are provided with a top cover.

14. A process for preparing at least one biological tissue in the slide chamber according to any of the claims 1 to 13 for optical examination characterized by placing the at least one biological tissue on the transparent closure member ( 30 ), combining the top member ( 10 ) and the transparent closure member ( 30 ) with an interleaved fluidic seal ( 20 );

attaching the base member ( 40 ) so that the interconnecting means ( 41 ) of the base member ( 40 ) are inserted into the openings ( 12 ) of the top member ( 10 ) and closing the slide chamber by lateral movement of the top member ( 10 ) against the transparent closure member ( 30 ).

15. The process according to claim 14 , characterized in that the at least one biological tissue is placed within at least two examination chambers.

16. The slide chamber of claim 1 , wherein the interconnecting means ( 41 ) is configured to create an increasing pressure (force) between the base member ( 40 ) and the top member ( 10 ) during the closing process, i.e. during lateral movement of the of the base member ( 40 ) against the top member ( 10 ).

17. The slide chamber of claim 16 , wherein the increasing pressure is generated by providing at least one of a tilted shape and a compressible fluidic seal ( 20 ) on at least one of the base member ( 40 ) and the openings ( 12 ), during lateral movement of the base member ( 40 ) against the top member ( 10 ).

Priority Claims (1)
EP 19163639 · Mar 19, 2019 · regional
Continuity (1)
Related Publication 20200298241A1 · Sep 24, 2020