Methods and devices for de novo oligonucleic acid assembly
Methods and devices are provided herein for surfaces for de novo nucleic acid synthesis which provide for low error rates. In addition, methods and devices are provided herein for increased nucleic acid mass yield resulting from de novo nucleic acid synthesis.
1. A method for polynucleotide synthesis, comprising:
a) providing predetermined sequences;
b) providing a device for polynucleotide synthesis, the device comprising:
i) a structure having a surface, wherein the structure comprises silicon dioxide;
ii) a plurality of recesses or posts on the surface, wherein each recess or post comprises:
1) a width length that is 6.8 nm to 500 nm,
2) a pitch length that is about twice the width length, and
3) a depth length that is about 60% to about 125% of the pitch length;
iii) a plurality of loci on the surface, wherein each locus has a diameter of 0.5 to 100 μm, wherein each locus comprises at least two of the plurality of recesses or posts; and
iv) a plurality of clusters on the surface, wherein each of the clusters comprise 50 to 500 loci and has a cross-section of 0.5 to 2 mm
c) synthesizing a plurality of non-identical polynucleotides at least 10 bases in length, wherein each of the non-identical polynucleotides extends from a different locus, wherein an aggregate deletion error rate is achieved without correcting errors.
2. The method of claim 1 , wherein the surface comprises a layer of silicon oxide.
3. The method of claim 1 , wherein the plurality of non-identical polynucleotides is 50 to 120 bases in length.
4. The method of claim 1 , wherein the plurality of non-identical polynucleotides comprises at least 5,000 polynucleotides.
5. The method of claim 1 , wherein the plurality of polynucleotides comprises at least 30,000 polynucleotides.
6. The method of claim 1 , wherein each locus comprises a molecule that binds to the surface and a nucleoside phosphoramidite.
7. The method of claim 6 , wherein the molecule that binds to the surface and the nucleoside phosphoramidite is a silane.
8. The method of claim 6 , wherein the molecule that binds to the surface and the nucleoside phosphoramidite is N-(3-triethoxysilylpropyl)-4-hydroxybutyramide (HAPS), 11-acetoxyundecyltriethoxysilane, n-decyltriethoxysilane, (3-aminopropyl)trimethoxysilane, (3-aminopropyl)triethoxysilane, 3-glycidoxypropyltrimethoxysilane, 3-iodo-propyltrimethoxysilane, or octylchlorosilane.
9. The method of claim 7 , wherein the silane is 3-glycidoxypropyltrimethoxysilane.
10. The method of claim 7 , wherein the silane is an aminosilane.
11. The method of claim 1 , wherein a region surrounding the plurality of loci comprises a molecule that binds to the surface and lacks a nucleoside phosphoramidite.
12. The method of claim 11 , wherein the molecule that binds to the surface and lacks the nucleoside phosphoramidite is a fluorosilane.
13. The method of claim 12 , wherein the fluorosilane is (tridecafluoro-1,1,2,2-tetrahydrooctyl)trichlorosilane.