Surfaces having reduced non-specific binding and antigenicity
Disclosed herein are compositions and methods for reducing the antigenicity of molecules. The antigenicity of a molecule may be reduced or eliminated by conjugating at least one branched polymer to the molecule to form a molecule-polymer conjugate. The branched polymer may include a backbone and a plurality of side chains, each side chain covalently attached to the backbone.
1. A surface having reduced antigenicity and non-specific binding comprising: a surface comprising a plurality of poly[oligo(ethylene glycol) methyl ether methacrylate] (POEGMA) polymers comprising a poly(methyl methacrylate) backbone and a plurality of side chains covalently attached to the backbone, each side chain comprising 1 to 9 ethylene glycol monomers repeated in tandem and terminating with an alkoxy-moiety.
2. The surface of claim 1 , wherein the surface is not reactive with anti-PEG antibodies in a subject.
3. The surface of claim 1 , wherein the surface does not bind proteins, lipids, or carbohydrates non-specifically.
4. The surface of claim 1 , wherein the surface is non-antigenic and does not induce an immune response.
5. The surface of claim 1 , wherein the side chain comprises 1 to 5 ethylene glycol monomers repeated in tandem.
6. The surface of claim 1 , wherein the side chain comprises 1 to 3 ethylene glycol monomers repeated in tandem.
7. The surface of claim 1 , wherein the side chain comprises 2 to 3 ethylene glycol monomers repeated in tandem.
8. The surface of claim 1 , wherein the side chain comprises 2 ethylene glycol monomers repeated in tandem.
9. The surface of claim 1 , wherein the side chain comprises 3 ethylene glycol monomers repeated in tandem.
10. The surface of claim 1 , wherein the alkoxy is methoxy, ethoxy, or propoxy.
11. The surface of claim 1 , wherein the alkoxy is methoxy.
12. The surface of claim 1 , wherein the POEGMA comprises monomers of ethylene glycol methyl ether methacrylate (EG-1-OMe).
13. The surface of claim 1 , wherein the POEGMA comprises monomers of di(ethylene glycol) methyl ether methacrylate (EG-2-OMe).
14. The surface of claim 1 , wherein the POEGMA comprises monomers of tri(ethylene glycol) methyl ether methacrylate (EG-3-OMe).
15. The surface of claim 1 , wherein the POEGMA comprises monomers of penta(ethylene glycol) methyl ether methacrylate (EG-5-OMe).
16. The surface of claim 1 , wherein the POEGMA comprises monomers of nona(ethylene glycol) methyl ether methacrylate (EG-9-OMe).
17. The surface of claim 1 , wherein the surface is a material or a biomolecule.
18. The surface of claim 1 , wherein the surface is a protein or a protein complex.
19. The surface of claim 1 , wherein the surface is a material comprising glass, plastic, metal, ceramic, textile, or paper.
20. A method for reducing antigenicity of and non-specific binding to a surface, the method comprising: affixing to a surface a plurality of poly[oligo(ethylene glycol) methyl ether methacrylate] (POEGMA) polymers comprising a poly(methyl methacrylate) backbone and a plurality of side chains covalently attached to the backbone, each side chain comprising 1 to 9 ethylene glycol monomers repeated in tandem and terminating with an alkoxy moiety.
21. The method of claim 20 , wherein the plurality of POEGMA polymers are affixed to the surface by contacting the surface with an initiator agent to form a macroinitiator; and incubating the macroinitiator with a monomer under conditions that permit free-radical polymerization and formation of a branched polymer to occur from the initiator agent.
22. The method of claim 20 , wherein the side chain comprises 1 to 5 ethylene glycol monomers repeated in tandem.
23. The method of claim 20 , wherein the side chain comprises 1 to 3 ethylene glycol monomers repeated in tandem.
24. The method of claim 20 , wherein the side chain comprises 2 to 3 ethylene glycol monomers repeated in tandem.
25. The method of claim 20 , wherein the side chain comprises 2 ethylene glycol monomers repeated in tandem.
26. The method of claim 20 , wherein the side chain comprises 3 ethylene glycol monomers repeated in tandem.
27. The method of claim 20 , wherein the alkoxy is methoxy, ethoxy, or propoxy.
28. The method of claim 20 , wherein the alkoxy is methoxy.
29. The method of claim 20 , wherein the POEGMA comprises monomers of ethylene glycol methyl ether methacrylate (EG-1-OMe).
30. The method of claim 20 , wherein the POEGMA comprises monomers of di(ethylene glycol) methyl ether methacrylate (EG-2-OMec).
31. The method of claim 20 , wherein the POEGMA comprises monomers of tri(ethylene glycol) methyl ether methacrylate (EG-3-OMe).
32. The method of claim 20 , wherein the POEGMA comprises monomers of penta(ethylene glycol) methyl ether methacrylate (EG-5-OMe).
33. The method of claim 20 , wherein the POEGMA comprises monomers of nona(ethylene glycol) methyl ether methacrylate (EG-9-OMe).
34. The method of claim 20 , wherein the surface is a material or a biomolecule.
35. The method of claim 20 , wherein the surface is a protein or a protein complex.
36. The method of claim 20 , wherein the surface is a protein and one or more POEGMA polymers is affixed to the polypeptide at the C-terminus, the N-terminus, or an internal amino acid of the polypeptide.
37. The method of claim 20 , wherein the surface is a material comprising glass, plastic, metal, ceramic, textile, or paper.
38. A surface coated with a plurality of POEGMA polymers by the method of claim 20 .
39. The surface of claim 38 , wherein the surface is a biomolecule or a material.
40. The surface of claim 38 , wherein the surface does not non-specifically bind proteins, carbohydrates, or lipids; does not induce an immune response, and is not reactive with anti-PEG antibodies.