Biological material collection and separation system and methods of use
View Patent ↗A system for collecting and separating biological material includes a centrifuge tube, a separation tube having an open bottom, a cap, a plug for temporarily sealing the open bottom of the separation tube, and a separation medium disposable within the centrifuge tube. The centrifuge tube and the separation tube sealingly and releasably couple to the cap, such that, when coupled, the separation tube is positioned within the centrifuge tube. The cap is configured to facilitate and/or regulate the introduction of air, gas, or other matter into the separation tube. When fully sealed, the separation tube may be placed under a vacuum condition, whereby a needle apparatus is used to facilitate introduction of matter into the separation tube. When the separation tube is positioned within the centrifuge tube, the bottom portion of the separation tube is submersed in the separation medium.
1. A method for separating and collecting a target material from a fluid sample comprising:
providing an apparatus comprising a centrifuge tube, a separation tube having an open bottom, and a cap, wherein the centrifuge tube and the separation tube are configured to sealingly and releasably couple to the cap such that a pre-determined length of the separation tube is positioned within the centrifuge tube, and a density separation medium disposable within the centrifuge tube and the separation tube, wherein a volume of the density separation medium is such that, when the separation tube and the centrifuge tube are coupled to the cap, the open bottom of the separation tube is submersed in the density separation medium;
depositing the fluid sample on top of the density separation medium inside of the separation tube;
centrifuging the apparatus with the fluid sample;
introducing air or gas into the separation tube utilizing a hollow needle operably coupled to a regulator configured to regulate a flow of matter and wherein the cap is configured to receive the needle therethrough; and halting air- or gas-flow into the separation tube such that a target material is separated from the fluid sample, and collecting the separated target material from the separation tube.
2. The method of claim 1 , wherein the needle is operably coupled to a regulator selected from the group consisting of: a syringe, a pump, and an air compressor.
3. The method of claim 1 , wherein the needle is punctured through the cap.
4. The method of claim 3 , wherein the cap is constructed of a flexible material.
5. The method of claim 1 , wherein the cap comprises a resealable valve or port integrated therewith configured to allow the needle to be inserted therethrough and reseal after the needle is removed from the cap.
6. The method of claim 1 , wherein a top of the cap comprises an aperture that opens into a cavity of the cap and further comprises a plug that is configured to releasably seal the aperture.
7. The method of claim 6 , wherein the needle is punctured through the plug.
8. The method of claim 7 , wherein the plug is constructed of a flexible material.
9. The method of claim 6 , wherein the plug comprises a resealable valve or port integrated therewith configured to allow the needle to be inserted therethrough and reseal after the needle is removed from the plug.
10. The method of claim 1 , wherein as air or gas is introduced into the separation tube through the needle, a corresponding volume of the fluid sample is controllably expelled through the open bottom of the separation tube.
11. The method of claim 1 , further comprising uncoupling the centrifuge tube from the cap once centrifugation is complete, wherein the target material is retained within the separation tube.
12. The method of claim 1 , further comprising re-introducing air or gas into the separation tube utilizing the hollow needle after halting the air- or gas-flow.
13. The method of claim 1 , wherein introducing air or gas into the separation tube results in a pressure inside of the separation tube being equal to or greater than an external atmospheric pressure surrounding the apparatus.
14. The method of claim 1 , wherein when coupled to the cap, an area between an exterior surface of the separation tube and an interior surface of the centrifuge tube is unobstructed along the pre-determined length.