Chimeric antigen receptor that binds HLA-DR and CAR-T cell
The present invention relates to an antigen-binding molecule comprising a heavy chain variable region comprising a heavy-chain complementarity-determining region 1 (HCDR1) comprising an amino acid sequence represented by Sequence No. 1, an HCDR2 comprising an amino acid sequence represented by Sequence No. 2, and an HCDR3 comprising an amino acid sequence represented by Sequence No. 3; a light-chain variable region comprising a light-chain complementarity-determining region 1 (LCDR1) comprising an amino acid sequence represented by Sequence No. 4, an LCDR2 comprising an amino acid sequence represented by Sequence No. 5, and an LCDR3 comprising an amino acid sequence represented by Sequence No. 6; wherein the antigen-binding molecule is a T cell receptor (TCR); and to a cell line expressing the same.
1. A nucleic acid molecule encoding an antigen binding molecule comprising a heavy-chain variable region of SEQ ID NO; 7 and a light-chain variable region of SEQ ID NO: 8, wherein the antigen-binding molecule is a chimeric antigen receptor (CAR).
2. An expression vector comprising the nucleic acid molecule of claim 1 .
3. A cell comprising the nucleic acid molecule of claim 1 .
4. The cell of claim 3 , wherein the cell is a T cell.
5. The cell of claim 4 , wherein the T cell is a CD8+ T cell, or a CD4+ T cell.
6. The cell of claim 4 , wherein the T cell is a chimeric antigen receptor-modified T cell (CAR-T).
7. A pharmaceutical composition for treating cancer, comprising at least one cell of claim 3 as a pharmaceutically effective ingredient.
8. The pharmaceutical composition of claim 7 , wherein the at least one cell comprises a T cell.
9. The pharmaceutical composition of claim 8 , wherein the at least one T cell comprises at least one CD8+ T cell, or at least one CD4+ T cell.
10. The pharmaceutical composition of claim 9 , wherein the at least one T cell comprises at least one CD8+ T cell.
11. The pharmaceutical composition of claim 8 , wherein the at least one T cell comprises at least one CD8+ T cell and at least one CD4+ T cell.
12. The pharmaceutical composition of claim 7 , further comprising a pharmaceutically acceptable carrier.
13. The pharmaceutical composition of claim 12 , further comprising one or more pharmaceutically acceptable excipients.
14. The pharmaceutical composition of claim 13 , wherein the one or more pharmaceutically acceptable excipients comprise human serum albumin, recombinant human albumin, gelatin, casein, alanine, arginine, glycine, betaine, histidine, glutamic acid, aspartic acid, cysteine, lysine, leucine, isoleucine, valine, methionine, phenylalanine, aspartame, fructose, maltose, galactose, glucose, D-mannose, sorbose; disaccharides such as lactose, sucrose, trehalose, cellobiose; polysaccharides such as raffinose, maltodextrin, dextran, starch, mannitol, xylitol, maltitol, lactitol, sorbitol, or myoinositol.
15. The pharmaceutical composition of claim 7 , wherein the pharmaceutical composition comprises an injection of sterile solution or suspension.
16. The pharmaceutical composition of claim 15 , wherein the injection is an intravenous injection, intraarterial injection, selective intraarterial injection, intramuscular injection, intraperitoneal injection, subcutaneous injection, intraventricular injection, intracranial injection, intramedullary injection.
17. The pharmaceutical composition of claim 16 , wherein the injection is an intravenous injection.
18. The pharmaceutical composition of claim 7 , wherein at least one cell comprises at least 1×10 6 cells/mL.
19. The pharmaceutical composition of claim 18 , wherein at least one cell comprises at least 5×10 6 cells/mL.
20. The pharmaceutical composition of claim 7 , wherein the cancer is esophageal adenocarcinoma, colorectal cancer, melanoma, ocular melanoma, small cell lung cancer, neuroblastoma, teratoma, fetal cancer, squamous cell carcinoma, head and neck squamous cell carcinoma, thymoma, lymphocytic leukemia, B-cell lymphoma, diffuse large B-cell lymphoma, leukemia, or acute myeloid leukemia.