Fat fragmentation device and method

1. A device for adipose tissue processing, microfragmentation and facilitation of mechanical separation of adipose derived stem cells (“ADSCs”), comprising:

an upper housing with an inlet,

a lower housing with an outlet,

a filter stack, and

a spiral flow effectuater,

wherein the upper housing and the lower housing are configured to join to form an enclosure that encloses the filter stack and the spiral flow effectuater,

wherein the spiral effectuater comprises spiral ridges and grooves and is configured to immediately attach to the filter stack and receive a flower of filtrate from the filter stack and generate a spiral flow of the adipose tissue, whereby minimizing a direct shock of the adipose tissue with walls of the filter stacker so as to promote enhanced mechanical separation with less trauma to cells of the adipose tissue, and

wherein the spiral effectuater is configured to be locked in a fixed position with respect to the upper housing, the lower housing, and the filter stack.

2. The device according to claim 1 , wherein the filter stack comprises at least one filter having multiple holes of a size that is the same or different, the size ranging from about 0.4 mm to about 3 mm.

3. The device according to claim 1 , wherein the filter stack comprises at least one filter having multiple holes of alternating sizes.

4. The device according to claim 1 , wherein the filter stack comprises a first filter, a second filter, and a third filter, where—

the first filter having multiple holes of a size that is about 2.2 mm;

the second filter having multiple holes of alternating sizes where one size that is about 1.8 mm and another size that is 1.35 mm; and

the third filter having multi holes of a size that is about 1.35 mm.

5. The device according to claim 1 , wherein the upper housing and lower housing are joined by a tongue and groove joint joined by ultrasonic welding.

6. The device according to claim 1 , wherein the filter stack comprises more than one filters, and the more than one filters are connected via rods or tubes.

7. The device according to claim 1 , wherein the inlet and outlet comprise a Luer lock thread.

8. The device according to claim 1 , wherein at least one of the upper housing, lower housing, the filter stack or the spiral flow effectuater is made from polycarbonate or stainless steel.

9. The device according to claim 1 , which is a disposable device.

10. A method of producing stromal vascular fraction of adipose tissue, comprising:

providing the device according to claim 1 ,

subjecting a volume of adipose tissue to fragmentation and separation by the device to generate a volume of fragmented adipose tissue, and

subject the fragmented adipose tissue to centrifuging to generate a volume of the stromal vascular fraction.

11. The method according to claim 10 , wherein the filter stack comprises at least one filter having multiple holes of a size that is the same or different, the size ranging from about 0.4 mm to about 3 mm.

12. The method according to claim 10 , wherein the filter stack comprises at least one filter having multiple holes of alternating sizes.

13. The method according to claim 10 , wherein the filter stack comprises a first filter, a second filter, and a third filter, where—

the first filter having multiple holes of a size that is about 2.2 mm;

the second filter having multiple holes of alternating sizes where one size that is about 1.8 mm and another size that is about 1.35 mm; and

the third filter having multi holes of a size that is the same ranging from about 1.35 mm to about 0.45 mm.

14. A graft, comprising a volume of the stromal vascular fraction (“SVF”) generated according to claim 10 .

15. The graft according to claim 11 , further comprising a pharmaceutically acceptable carrier.

16. The graft according to claim 11 , further comprising a volume of adipose tissue.