Method for producing washing enzyme having protease resistance
Provided is a method for producing washing enzyme having protease resistance. According to the method, the washing enzyme having resistance to protease is obtained by carrying out fusion expression on a gene of the washing enzyme with the gene of a protease inhibitory peptide, thereby facilitating maintaining the stability of various enzyme components in an enzyme-containing detergent, and improving the use effect of the detergent.
1. A laundry enzyme with protease resistance, which is a fusion protein of a laundry enzyme and a protease inhibitory peptide; wherein the laundry enzyme is selected from the group consisting of cellulase, lipase, cutinase, amylase, glucoamylase, pectinase, mannanase, arabinase, galactosidase, xylanase, laccase and peroxidase; and
the protease inhibitory peptide is selected from the group consisting of Streptomyces subtilisin inhibitor (SSI) from Streptomyces sp., protease inhibitory peptide PCL from Barley, protease inhibitor marinostatin (MST) from Alteromonas , and a mixture thereof.
2. The laundry enzyme with protease resistance according to claim 1 , wherein the laundry enzyme and the protease inhibitory peptide of the fusion protein are fused covalently.
3. The laundry enzyme with protease resistance according to claim 1 , wherein the laundry enzyme is cellulase or lipase, and the protease inhibitory peptide is selected from the group consisting of Streptomyces subtilisin inhibitor (SSI) from Streptomyces sp., protease inhibitory peptide PCL from Barley, and protease inhibitor marinostatin (MST) from Alteromonas.
4. The laundry enzyme with protease resistance according to claim 3 , wherein the laundry enzyme is cellulase, and the protease inhibitory peptide is selected from the group consisting of protease inhibitory peptide PCL from Barley, and protease inhibitor marinostatin (MST) from Alteromonas.
5. The laundry enzyme with protease resistance according to claim 1 , wherein the laundry enzyme is cellulase.
6. The laundry enzyme with protease resistance according to claim 1 , wherein the laundry enzyme is lipase.
7. The laundry enzyme with protease resistance according to claim 1 , wherein the protease inhibitory peptide is fused to the laundry enzyme in N-terminus, C-terminus or middle.