Fluorescence quencher polymer conjugates and uses thereof
The invention provides chemical conjugates comprising a plurality of fluorescence quenching moieties conjugated to polymer and the use of such conjugates to quench the emission of fluorescence from emitters including macromolecular fluorescent emitters such as phycoerythrin.
1. A fluorescence quenching compound, comprising:
a plurality of discrete fluorescence quenching moieties; and a polyamine,
wherein each of the plurality of discrete fluorescence quenching moieties is chemically conjugated to a discrete site of the polyamine.
2. The compound of claim 1 , wherein the polyamine is a branched polyamine.
3. The compound of claim 1 , wherein the polyamine comprises branched polyethylenimine.
4. The compound of claim 3 , wherein the polyamine is a branched polyethylenimine.
5. The compound of claim 3 , wherein the branched polyethylenimine has a molecular weight of Mw 10,000 or greater.
6. The compound of claim 3 , wherein the branched polyethylenimine has a molecular weight of Mw 10,000 or lower.
7. The compound of claim 1 ,
wherein the plurality of discrete fluorescence quenching moieties comprises
as a moiety.
8. The compound of claim 1 ,
wherein the plurality of fluorescence quenching moieties comprises
as a moiety.
9. The compound of claim 1 ,
wherein the plurality of fluorescence quenching moieties comprises
as a moiety.
10. The compound of claim 1 ,
wherein the plurality of fluorescence quenching moieties comprises
as a moiety.
11. The compound of claim 1 ,
wherein the plurality of fluorescence quenching moieties comprises
as a moiety.
12. The compound of claim 1 ,
wherein the plurality of fluorescence quenching moieties comprises
as a moiety.
13. The compound of claim 1 ,
wherein the plurality of fluorescence quenching moieties comprises
as a moiety.
14. The compound of claim 1 ,
wherein the plurality of fluorescence quenching moieties comprises
as a moiety.
15. The compound of claim 1 ,
wherein the plurality of fluorescence quenching moieties comprises
as a moiety.