Organomimetic devices and methods of use and manufacturing thereof
An organomimetic device includes a microfluidic device that can be used to culture cells in its microfluidic channels. The organomimetic device can be part of dynamic system that can apply mechanical forces to the cells by modulating the microfluidic device and the flow of fluid through the microfluidic channels. The membrane in the organomimetic device can be modulated mechanically via pneumatic means and/or mechanical means. The organomimetic device can be manufactured by the fabrication of individual components separately, for example, as individual layers that can be subsequently laminated together.
1. A microfluidic device comprising:
a) a central microchannel having a longitudinal axis, an inlet port, an outlet port, and a plurality of cut-outs that extend transverse to said longitudinal axis of said central microchannel, said plurality of cut-outs are not fluidically connected to said central microchannel, and are positioned between said inlet and said outlet ports; and
b) a plurality of opposing connection elements fastened to a first and opposing second location of said microfluidic device, said opposing connection elements physically connected to a mechanical stretch actuator configured to impart motion that is transferred to a portion of the microfluidic device between said first location and the opposing second location.
2. The device of claim 1 , wherein said central microchannel of said device is disposed in a first microchannel layer comprising a substantially rigid layer.
3. The device of claim 1 , wherein said device comprises a polymer selected from the group consisting of styrene-ethylene-butylene-styrene (SEES), a SEBS/polypropylene combination, and a cyclic polyolefin.
4. The device of claim 1 , wherein said central microchannel of said device is disposed in a first microchannel layer comprising a material selected from the group consisting of an extracellular matrix polymer, gel, biopolymer and a scaffold.
5. The device of claim 1 , further comprising a membrane which divides said central microchannel into first and second microchannels.
6. The device of claim 5 , further comprising a second microchannel layer.
7. The device of claim 5 , wherein at least a portion of said central microchannel is aligned with at least a portion of said second microchannel.
8. The device of claim 1 , wherein said device is comprised of one or a mixture of biocompatible materials each having a thickness, and wherein said plurality of cut-outs define a void extending through each thickness of each of said one or a mixture of biocompatible materials.