Separation and identification of myricetin-3-ogalactoside from casuarina glauca
The disclosure of the present patent application relates generally to the separation and identification of myricetin-3-O-galactoside from Casuarina glauca Sieber branchlets.
1. A process for separation and identification of myricetin-3-O-galactoside comprising:
providing fresh branchlets of a Casuarina glauca plant;
shade drying and powdering the fresh branchlets to obtain a Casuarina glauca plant powder;
soaking 300 g of the Casuarina glauca plant powder with 70% ethyl alcohol to obtain a Casuarina glauca plant extract;
concentrating the Casuarina glauca plant extract to obtain a residue;
dissolving the residue to obtain an aqueous mixture;
mixing and sonicating the aqueous mixture;
centrifuging the aqueous mixture to obtain a supernatant;
separating myricetin-3-O-galactoside from the supernatant; and
wherein the soaking step is repeated three times in successive portions of 1 L of the 70% ethyl alcohol.
2. The process of claim 1 , wherein the plant is Casuarina glauca Sieber.
3. The process of claim 1 , wherein the supernatant is about 1 ml of a mobile phase solution comprising water:methanol:acetonitrile (50:25:25).
4. The process of claim 1 , wherein the mixing and sonicating step comprises:
vortexing the aqueous mixture for about 2 minutes; and
subjecting the aqueous mixture to ultrasonication for about 10 minutes.