IP Library › Granted Patent US 12,188,014
Granted Patent B1
US 12,188,014 · App. 16/434,102 · Granted Jan 7, 2025

Compositions and methods for nucleic acid processing using blocking agents

Inventors: Andrew D. Price (Hayward, CA); Geoffrey McDermott (Livermore, CA)
Assignee: 10X GENOMICS, INC.
C12N15/1096C12N9/22C12N15/11C12Q1/6806C12N2310/20C12N2800/80
View Patent ↗
Loading inventors, assignments & file history…
Monitor This Case
Get email alerts when status or documents change.
Order Certified Copies
Most orders are placed with the USPTO same day — all within 24 business hours.
Order via The Patent Place →
Pre-filled with this patent's details
Quick Facts
Patent No.
US 12,188,014
App. No.
16/434,102
Granted
Jan 7, 2025
Kind
B1
Abstract

Methods and systems for sample preparation techniques that prevent inhibition of reactions such as due to the presence of longer oligonucleotides are presented herein. The methods and systems provided herein allow amplification (e.g., whole genome amplification) and sequencing of chromatin accessible regions of single cells.

Claims (34)

1. A method for nucleic acid processing, comprising:

(a) providing a reaction mixture comprising:

(i) a transposase enzyme,

(ii) a first nucleic acid molecule comprising a target sequence,

(iii) a second nucleic acid molecule comprising a transposon end sequence, and

(iv) a third nucleic acid molecule comprising: (1) a first region comprising a blocking agent, wherein said blocking agent is not a uracil binding protein, and wherein said blocking agent prevents said transposase enzyme from interacting with said first region of said third nucleic acid molecule; and (2) a second region comprising a functional sequence capable of coupling to said first nucleic acid molecule;

(b) contacting said first nucleic acid molecule, said second nucleic acid molecule, and said transposase enzyme to generate a fourth nucleic acid molecule comprising said target sequence and said transposon end sequence of said second nucleic acid molecule; and

(c) contacting said third nucleic acid molecule and said fourth nucleic acid molecule to generate a fifth nucleic acid molecule comprising said target sequence, said transposon end sequence, and a sequence of said third nucleic acid molecule.

2. The method of claim 1 , further comprising partitioning said reaction mixture into a partition, wherein said partition is selected from the group consisting of an aqueous droplet in an emulsion, a well, and an aqueous droplet in an emulsion trapped in a well.

3. The method of claim 1 , wherein said functional sequence is capable of hybridizing to said first nucleic acid molecule or said fourth nucleic acid molecule.

4. The method of claim 3 , wherein said functional sequence capable of hybridizing to said first nucleic acid molecule or said fourth nucleic acid molecule is a random N-mer sequence.

5. The method of claim 3 , wherein said functional sequence capable of hybridizing to said first nucleic acid molecule or said fourth nucleic acid molecule is a targeted N-mer sequence.

6. The method of claim 3 , wherein said functional sequence capable of hybridizing to said first nucleic acid molecule or said fourth nucleic acid molecule is a poly-T sequence.

7. The method of claim 1 , further comprising generating said fifth nucleic acid molecule by a nucleic acid extension reaction or an amplification reaction.

8. The method of claim 1 , further comprising generating said fifth nucleic acid molecule by a reverse transcription reaction.

9. The method of claim 1 , further comprising generating said fifth nucleic acid molecule by a ligation reaction.

10. The method of claim 1 , wherein said third nucleic acid molecule further comprises a biotinylated nucleotide, and wherein said blocking agent is said biotinylated nucleotide or a biotin-binding agent.

11. The method of claim 1 , wherein said third nucleic acid molecule further comprises an azide modified nucleotide, and wherein said blocking agent is an azide-binding blocking agent.

12. The method of claim 1 , wherein said third nucleic acid molecule further comprises a modified nucleotide, and wherein said blocking agent is an antibody specific to said modified nucleotide.

13. The method of claim 1 , wherein said third nucleic acid molecule further comprises a sequence capable of recognition by a nuclease, and wherein said blocking agent is an inactivated nuclease.

14. The method of claim 13 , wherein said inactivated nuclease is a restriction endonuclease.

15. The method of claim 1 , wherein said reaction mixture further comprises a plurality of guide ribonucleic acid molecules (gRNAs) targeted to a sequence of said second nucleic acid molecule, wherein said blocking agent is a modified clustered regularly interspaced short palindromic (CRISPR) associated (Cas) nuclease, and wherein said modified Cas nuclease does not have nuclease activity.

16. The method of claim 15 , wherein said modified Cas nuclease is a modified Cas9 protein.

17. The method of claim 1 , wherein said blocking agent is a DNA binding protein or a DNA binding domain of a DNA binding protein.

18. The method of claim 17 , wherein said DNA binding protein is a transcription activator-like (TAL) effector or zinc finger protein.

19. The method of claim 1 , further comprising removing or releasing said blocking agent from said third nucleic acid molecule.

20. The method of claim 19 , wherein said blocking agent is removed or released from said third nucleic acid molecule subsequent to (b).

21. The method of claim 1 , wherein said third nucleic acid molecule further comprises one or more additional functional sequences selected from the group consisting of an adapter sequence, a primer or primer binding sequence, a barcode sequence, a unique molecular index (UMI), and a sequence configured to attach to a flow cell of a sequencer.

22. The method of claim 21 , wherein said primer or primer binding sequence is a sequencing primer or sequencing primer binding sequence.

23. The method of claim 1 , wherein said third nucleic acid molecule is coupled to a bead.

24. The method of claim 23 , wherein said third nucleic acid molecule is releasable from said bead upon application of a stimulus.

25. The method of claim 23 , wherein said bead is a gel bead.

26. The method of claim 25 , wherein said gel bead is degradable upon application of a stimulus.

27. The method of claim 25 , wherein said third nucleic acid molecule is releasable from said gel bead upon application of a stimulus.

Assignments (1)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Aug 16, 2019
From: PRICE, ANDREW D.; MCDERMOTT, GEOFFREY
To: 10X GENOMICS, INC.
Reel/Frame 050073/0724 →
Continuity (1)
Provisional Application 62703303 · Jul 25, 2018
References Cited (400)
US 4124638A · Hansen · 1978 [cited by applicant]
US 5185099A · Delpuech et al. · 1993 [cited by applicant]
US 5270183A · Corbett et al. · 1993 [cited by applicant]
US 5478893A · Ghosh et al. · 1995 [cited by applicant]
US 5736330A · Fulton · 1998 [cited by applicant]
US 5756334A · Perler et al. · 1998 [cited by applicant]
US 5846719A · Brenner et al. · 1998 [cited by applicant]
US 5900481A · Lough et al. · 1999 [cited by applicant]
US 5942609A · Hunkapiller et al. · 1999 [cited by applicant]
US 5994056A · Higuchi · 1999 [cited by applicant]
US 6033880A · Haff et al. · 2000 [cited by applicant]
US 6057149A · Burns et al. · 2000 [cited by applicant]
US 6123798A · Gandhi et al. · 2000 [cited by applicant]
US 6171850B1 · Nagle et al. · 2001 [cited by applicant]
US 6172218B1 · Brenner · 2001 [cited by applicant]
US 6176962B1 · Soane et al. · 2001 [cited by applicant]
US 6306590B1 · Mehta et al. · 2001 [cited by applicant]
US 6327410B1 · Walt et al. · 2001 [cited by applicant]
US 6379929B1 · Burns et al. · 2002 [cited by applicant]
US 6406848B1 · Bridgham et al. · 2002 [cited by applicant]
US 6409832B2 · Weigl et al. · 2002 [cited by applicant]
US 6492118B1 · Abrams et al. · 2002 [cited by applicant]
US 6524456B1 · Ramsey et al. · 2003 [cited by applicant]
US 6586176B1 · Trnovsky et al. · 2003 [cited by applicant]
US 6632655B1 · Mehta et al. · 2003 [cited by applicant]
US 6915679B2 · Chien et al. · 2005 [cited by applicant]
US 7041481B2 · Anderson et al. · 2006 [cited by applicant]
US 7115400B1 · Adessi et al. · 2006 [cited by applicant]
US 7129091B2 · Ismagilov et al. · 2006 [cited by applicant]
US 7268167B2 · Higuchi et al. · 2007 [cited by applicant]
US 7282370B2 · Bridgham et al. · 2007 [cited by applicant]
US 7294503B2 · Quake et al. · 2007 [cited by applicant]
US 7323305B2 · Leamon et al. · 2008 [cited by applicant]
US 7544473B2 · Brenner · 2009 [cited by applicant]
US 7622076B2 · Davies et al. · 2009 [cited by applicant]
US 7622280B2 · Holliger et al. · 2009 [cited by applicant]
US 7645596B2 · Williams et al. · 2010 [cited by applicant]
US 7708949B2 · Stone et al. · 2010 [cited by applicant]
US 7772287B2 · Higuchi et al. · 2010 [cited by applicant]
US 7776927B2 · Chu et al. · 2010 [cited by applicant]
US 7842457B2 · Berka et al. · 2010 [cited by applicant]
US 7910354B2 · Drmanac et al. · 2011 [cited by applicant]
US 7927797B2 · Nobile et al. · 2011 [cited by applicant]
US 7960104B2 · Drmanac et al. · 2011 [cited by applicant]
US 7968287B2 · Griffiths et al. · 2011 [cited by applicant]
US 8053192B2 · Bignell et al. · 2011 [cited by applicant]
US 8133719B2 · Drmanac et al. · 2012 [cited by applicant]
US 8168385B2 · Brenner · 2012 [cited by applicant]
US 8268564B2 · Roth et al. · 2012 [cited by applicant]
US 8273573B2 · Ismagilov et al. · 2012 [cited by applicant]
US 8298767B2 · Brenner et al. · 2012 [cited by applicant]
US 8304193B2 · Ismagilov et al. · 2012 [cited by applicant]
US 8318433B2 · Brenner · 2012 [cited by applicant]
US 8329407B2 · Ismagilov et al. · 2012 [cited by applicant]
US 8592150B2 · Drmanac et al. · 2013 [cited by applicant]
US 8658430B2 · Miller et al. · 2014 [cited by applicant]
US 8822148B2 · Ismagliov et al. · 2014 [cited by applicant]
US 8835358B2 · Fodor et al. · 2014 [cited by applicant]
US 8871444B2 · Griffiths et al. · 2014 [cited by applicant]
US 8889083B2 · Ismagilov et al. · 2014 [cited by applicant]
US 8975302B2 · Light et al. · 2015 [cited by applicant]
US 9012390B2 · Holtze et al. · 2015 [cited by applicant]
US 9029083B2 · Griffiths et al. · 2015 [cited by applicant]
US 9029085B2 · Agresti et al. · 2015 [cited by applicant]
US 9085798B2 · Chee · 2015 [cited by applicant]
US 9089844B2 · Hiddessen et al. · 2015 [cited by applicant]
US 9126160B2 · Ness et al. · 2015 [cited by applicant]
US 9156010B2 · Colston et al. · 2015 [cited by applicant]
US 9194861B2 · Hindson et al. · 2015 [cited by applicant]
US 9216392B2 · Hindson et al. · 2015 [cited by applicant]
US 9238206B2 · Rotem et al. · 2016 [cited by applicant]
US 9266104B2 · Link · 2016 [cited by applicant]
US 9290808B2 · Fodor et al. · 2016 [cited by applicant]
US 9328382B2 · Drmanac et al. · 2016 [cited by applicant]
US 9347059B2 · Saxonov · 2016 [cited by applicant]
US 9371598B2 · Chee · 2016 [cited by applicant]
US 9388465B2 · Hindson et al. · 2016 [cited by applicant]
US 9417190B2 · Hindson et al. · 2016 [cited by applicant]
US 9486757B2 · Romanowsky et al. · 2016 [cited by applicant]
US 9498761B2 · Holtze et al. · 2016 [cited by applicant]
US 9500664B2 · Ness et al. · 2016 [cited by applicant]
US 9567631B2 · Hindson et al. · 2017 [cited by applicant]
US 9593365B2 · Frisen et al. · 2017 [cited by applicant]
US 9623384B2 · Hindson et al. · 2017 [cited by applicant]
US 9637799B2 · Fan et al. · 2017 [cited by applicant]
US 9644204B2 · Hindson et al. · 2017 [cited by applicant]
US 9689024B2 · Hindson et al. · 2017 [cited by applicant]
US 9694361B2 · Bharadwaj et al. · 2017 [cited by applicant]
US 9701998B2 · Hindson et al. · 2017 [cited by applicant]
US 9764322B2 · Hiddessen et al. · 2017 [cited by applicant]
US 9824068B2 · Wong · 2017 [cited by applicant]
US 9868979B2 · Chee et al. · 2018 [cited by applicant]
US 9879313B2 · Chee et al. · 2018 [cited by applicant]
US 9946577B1 · Stafford et al. · 2018 [cited by applicant]
US 9951386B2 · Hindson et al. · 2018 [cited by applicant]
US 9957558B2 · Leamon et al. · 2018 [cited by applicant]
US 9975122B2 · Masquelier et al. · 2018 [cited by applicant]
US 10011872B1 · Belgrader et al. · 2018 [cited by applicant]
US 10017759B2 · Kaper et al. · 2018 [cited by applicant]
US 10030261B2 · Frisen et al. · 2018 [cited by applicant]
US 10059989B2 · Giresi et al. · 2018 [cited by applicant]
US 10221436B2 · Hardenbol et al. · 2019 [cited by applicant]
US 10221442B2 · Hindson et al. · 2019 [cited by applicant]
US 10253364B2 · Hindson et al. · 2019 [cited by applicant]
US 10273541B2 · Hindson et al. · 2019 [cited by applicant]
US 10323279B2 · Hindson et al. · 2019 [cited by applicant]
US 10347365B2 · Wong et al. · 2019 [cited by applicant]
US 10357771B2 · Bharadwaj et al. · 2019 [cited by applicant]
US 10395758B2 · Schnall-Levin · 2019 [cited by applicant]
US 10400280B2 · Hindson et al. · 2019 [cited by applicant]
US 10428326B2 · Belhocine et al. · 2019 [cited by applicant]
US 10533221B2 · Hindson et al. · 2020 [cited by applicant]
US 10544413B2 · Bharadwaj et al. · 2020 [cited by applicant]
US 10549279B2 · Bharadwaj et al. · 2020 [cited by applicant]
US 10590244B2 · Delaney et al. · 2020 [cited by applicant]
US 10745742B2 · Bent et al. · 2020 [cited by applicant]
US 10752949B2 · Hindson et al. · 2020 [cited by applicant]
US 10774374B2 · Frisen et al. · 2020 [cited by applicant]
US 10815525B2 · Lucero et al. · 2020 [cited by applicant]
US 10829815B2 · Bharadwaj et al. · 2020 [cited by applicant]
US 10837047B2 · Delaney et al. · 2020 [cited by applicant]
US 10844372B2 · Belhocine · 2020 [cited by examiner]
US 10874997B2 · Weitz et al. · 2020 [cited by applicant]
US 10995333B2 · Pfeiffer · 2021 [cited by applicant]
US 11459607B1 · Terry et al. · 2022 [cited by applicant]
US 11467153B2 · Belhocine et al. · 2022 [cited by applicant]
US 11655499B1 · Pfeiffer · 2023 [cited by applicant]
US 20020005354A1 · Spence et al. · 2002 [cited by applicant]
US 20020051971A1 · Stuelpnagel et al. · 2002 [cited by applicant]
US 20020058332A1 · Quake et al. · 2002 [cited by applicant]
US 20020092767A1 · Bjornson et al. · 2002 [cited by applicant]
US 20020119455A1 · Chan · 2002 [cited by applicant]
US 20020127736A1 · Chou et al. · 2002 [cited by applicant]
US 20030036206A1 · Chien et al. · 2003 [cited by applicant]
US 20030075446A1 · Culbertson et al. · 2003 [cited by applicant]
US 20030124509A1 · Kenis et al. · 2003 [cited by applicant]
US 20030215862A1 · Parce et al. · 2003 [cited by applicant]
US 20040068019A1 · Higuchi et al. · 2004 [cited by applicant]
US 20050130188A1 · Walt et al. · 2005 [cited by applicant]
US 20050250147A1 · Macevicz · 2005 [cited by applicant]
US 20050266582A1 · Modlin et al. · 2005 [cited by applicant]
US 20050287572A1 · Mathies et al. · 2005 [cited by applicant]
US 20060177832A1 · Brenner · 2006 [cited by applicant]
US 20060275782A1 · Gunderson et al. · 2006 [cited by applicant]
US 20070020640A1 · McCloskey et al. · 2007 [cited by applicant]
US 20070042419A1 · Barany et al. · 2007 [cited by applicant]
US 20070172873A1 · Brenner et al. · 2007 [cited by applicant]
US 20070190543A1 · Livak · 2007 [cited by applicant]
US 20070196397A1 · Torii et al. · 2007 [cited by applicant]
US 20070264320A1 · Lee et al. · 2007 [cited by applicant]
US 20080056948A1 · Dale et al. · 2008 [cited by applicant]
US 20080166720A1 · Hsieh et al. · 2008 [cited by applicant]
US 20080242560A1 · Gunderson et al. · 2008 [cited by applicant]
US 20090011943A1 · Drmanac et al. · 2009 [cited by applicant]
US 20090047713A1 · Handique · 2009 [cited by applicant]
US 20090099041A1 · Church et al. · 2009 [cited by applicant]
US 20090131543A1 · Weitz et al. · 2009 [cited by applicant]
US 20090148961A1 · Luchini et al. · 2009 [cited by applicant]
US 20090155563A1 · Petsev et al. · 2009 [cited by applicant]
US 20090202984A1 · Cantor · 2009 [cited by applicant]
US 20090235990A1 · Beer · 2009 [cited by applicant]
US 20090269248A1 · Falb et al. · 2009 [cited by applicant]
US 20090286687A1 · Dressman et al. · 2009 [cited by applicant]
US 20100035254A1 · Williams · 2010 [cited by applicant]
US 20100069263A1 · Shendure et al. · 2010 [cited by applicant]
US 20100086914A1 · Bentley et al. · 2010 [cited by applicant]
US 20100105112A1 · Holtze et al. · 2010 [cited by applicant]
US 20100105866A1 · Fraden et al. · 2010 [cited by applicant]
US 20100184928A1 · Kumacheva · 2010 [cited by applicant]
US 20100216153A1 · Lapidus et al. · 2010 [cited by applicant]
US 20100248991A1 · Roesler et al. · 2010 [cited by applicant]
US 20100304982A1 · Hinz et al. · 2010 [cited by applicant]
US 20110217736A1 · Hindson · 2011 [cited by applicant]
US 20110305761A1 · Shum et al. · 2011 [cited by applicant]
US 20120071331A1 · Casbon et al. · 2012 [cited by applicant]
US 20120172259A1 · Rigatti et al. · 2012 [cited by applicant]
US 20120190032A1 · Ness et al. · 2012 [cited by applicant]
US 20120196288A1 · Beer · 2012 [cited by applicant]
US 20120219947A1 · Yurkovetsky et al. · 2012 [cited by applicant]
US 20130028812A1 · Prieto et al. · 2013 [cited by applicant]
US 20130109575A1 · Kleinschmidt et al. · 2013 [cited by applicant]
US 20130288925A1 · Janulaitis et al. · 2013 [cited by applicant]
US 20140065234A1 · Shum et al. · 2014 [cited by applicant]
US 20140155295A1 · Hindson et al. · 2014 [cited by applicant]
US 20140221239A1 · Carman et al. · 2014 [cited by applicant]
US 20140272996A1 · Bemis · 2014 [cited by applicant]
US 20140274740A1 · Srinivasan et al. · 2014 [cited by applicant]
US 20140302503A1 · Lowe et al. · 2014 [cited by applicant]
US 20140338753A1 · Sperling et al. · 2014 [cited by applicant]
US 20140378322A1 · Hindson · 2014 [cited by examiner]
US 20140378345A1 · Hindson et al. · 2014 [cited by applicant]
US 20150066385A1 · Schnall-Levin et al. · 2015 [cited by applicant]
US 20150220532A1 · Wong · 2015 [cited by applicant]
US 20150267191A1 · Steelman et al. · 2015 [cited by applicant]
US 20150292988A1 · Bharadwaj et al. · 2015 [cited by applicant]
US 20150361418A1 · Reed · 2015 [cited by applicant]
US 20150376605A1 · Jarosz et al. · 2015 [cited by applicant]
US 20150376608A1 · Kaper et al. · 2015 [cited by applicant]
US 20150376609A1 · Hindson et al. · 2015 [cited by applicant]
US 20150376700A1 · Schnall-Levin et al. · 2015 [cited by applicant]
US 20150379196A1 · Schnall-Levin et al. · 2015 [cited by applicant]
US 20160008778A1 · Weitz et al. · 2016 [cited by applicant]
US 20160024558A1 · Hardenbol et al. · 2016 [cited by applicant]
US 20160024572A1 · Shishkin et al. · 2016 [cited by applicant]
US 20160053253A1 · Salathia et al. · 2016 [cited by applicant]
US 20160059204A1 · Hindson et al. · 2016 [cited by applicant]
US 20160060621A1 · Agresti et al. · 2016 [cited by applicant]
US 20160122753A1 · Mikkelsen et al. · 2016 [cited by applicant]
US 20160122817A1 · Jarosz et al. · 2016 [cited by applicant]
US 20160203196A1 · Schnall-Levin et al. · 2016 [cited by applicant]
US 20160232291A1 · Kyriazopoulou-Panagiotopoulou et al. · 2016 [cited by applicant]
US 20160244809A1 · Belgrader et al. · 2016 [cited by applicant]
US 20160281160A1 · Jarosz et al. · 2016 [cited by applicant]
US 20160289769A1 · Schwartz et al. · 2016 [cited by applicant]
US 20160314242A1 · Schnall-Levin et al. · 2016 [cited by applicant]
US 20160348093A1 · Price et al. · 2016 [cited by applicant]
US 20160376605A1 · Konzak et al. · 2016 [cited by applicant]
US 20170016041A1 · Greenfield et al. · 2017 [cited by applicant]
US 20170128937A1 · Hung et al. · 2017 [cited by applicant]
US 20170144161A1 · Hindson et al. · 2017 [cited by applicant]
US 20170145476A1 · Ryvkin et al. · 2017 [cited by applicant]
US 20170159109A1 · Zheng et al. · 2017 [cited by applicant]
US 20170235876A1 · Jaffe et al. · 2017 [cited by applicant]
US 20170260584A1 · Zheng et al. · 2017 [cited by applicant]
US 20180030515A1 · Regev et al. · 2018 [cited by applicant]
US 20180071695A1 · Weitz et al. · 2018 [cited by applicant]
US 20180080075A1 · Brenner et al. · 2018 [cited by applicant]
US 20180105808A1 · Mikkelsen et al. · 2018 [cited by applicant]
US 20180155767A1 · Myers · 2018 [cited by examiner]
US 20180216162A1 · Belhocine et al. · 2018 [cited by applicant]
US 20180312822A1 · Lee et al. · 2018 [cited by applicant]
US 20180312873A1 · Zheng · 2018 [cited by applicant]
US 20180334670A1 · Bharadwaj et al. · 2018 [cited by applicant]
US 20180340169A1 · Belhocine et al. · 2018 [cited by applicant]
US 20180371545A1 · Wong et al. · 2018 [cited by applicant]
US 20190060890A1 · Bharadwaj et al. · 2019 [cited by applicant]
US 20190060904A1 · Bharadwaj et al. · 2019 [cited by applicant]
US 20190060905A1 · Bharadwaj et al. · 2019 [cited by applicant]
US 20190064173A1 · Bharadwaj et al. · 2019 [cited by applicant]
US 20190071656A1 · Chang et al. · 2019 [cited by applicant]
US 20190085391A1 · Hindson et al. · 2019 [cited by applicant]
US 20190100632A1 · Delaney et al. · 2019 [cited by applicant]
US 20190127731A1 · McDermott · 2019 [cited by applicant]
US 20190134633A1 · Bharadwaj et al. · 2019 [cited by applicant]
US 20190136316A1 · Hindson et al. · 2019 [cited by applicant]
US 20190153532A1 · Bharadwaj et al. · 2019 [cited by applicant]
US 20190176152A1 · Bharadwaj et al. · 2019 [cited by applicant]
US 20190177800A1 · Boutet et al. · 2019 [cited by applicant]
US 20190249226A1 · Bent et al. · 2019 [cited by applicant]
US 20190323088A1 · Boutet et al. · 2019 [cited by applicant]
US 20190345636A1 · McDermott et al. · 2019 [cited by applicant]
US 20190352717A1 · Schnall-Levin · 2019 [cited by applicant]
US 20190367997A1 · Bent et al. · 2019 [cited by applicant]
US 20190376118A1 · Belhocine et al. · 2019 [cited by applicant]
US 20200002763A1 · Belgrader et al. · 2020 [cited by applicant]
US 20200005902A1 · Mellen et al. · 2020 [cited by applicant]
US 20200032335A1 · Alvarado Martinez · 2020 [cited by applicant]
US 20200033237A1 · Hindson et al. · 2020 [cited by applicant]
US 20200033366A1 · Alvarado Martinez · 2020 [cited by applicant]
US 20200056223A1 · Bell · 2020 [cited by applicant]
US 20200105373A1 · Zheng · 2020 [cited by applicant]
US 20200263232A1 · Bell et al. · 2020 [cited by applicant]
US 20210190770A1 · Delaney et al. · 2021 [cited by applicant]
US 20210270703A1 · Abousoud · 2021 [cited by applicant]
US 20220162671A1 · Pfeiffer et al. · 2022 [cited by applicant]
US 20220403375A1 · Martinez · 2022 [cited by applicant]
US 20230167496A1 · Bava · 2023 [cited by applicant]
US 20240002914A1 · Pfeiffer et al. · 2024 [cited by applicant]
EP 1019496B1 · 2004 [cited by applicant]
EP 1841879A2 · 2007 [cited by applicant]
EP 1841879A4 · 2009 [cited by applicant]
EP 1967592B1 · 2010 [cited by applicant]
EP 2540389A1 · 2013 [cited by applicant]
EP 2635679B1 · 2017 [cited by applicant]
GB 2097692A · 1982 [cited by applicant]
GB 2097692B · 1985 [cited by applicant]
WO WO8402000 · 1984 [cited by applicant]
WO WO9530782 · 1995 [cited by applicant]
WO WO9952708 · 1999 [cited by applicant]
WO WO2000008212A1 · 2000 [cited by applicant]
WO WO2001002850A1 · 2001 [cited by applicant]
WO WO0114589A2 · 2001 [cited by applicant]
WO WO0189787A2 · 2001 [cited by applicant]
WO WO0190418A1 · 2001 [cited by applicant]
WO WO2001089787A9 · 2001 [cited by applicant]
WO WO2004002627A2 · 2004 [cited by applicant]
WO WO2004065617A2 · 2004 [cited by applicant]
WO WO2004069849A2 · 2004 [cited by applicant]
WO WO2004091763A2 · 2004 [cited by applicant]
WO WO2004069849A3 · 2005 [cited by applicant]
WO WO2005021151A1 · 2005 [cited by applicant]
WO WO2005049787A2 · 2005 [cited by applicant]
WO WO2005082098A2 · 2005 [cited by applicant]
WO WO2005082098A3 · 2005 [cited by applicant]
WO WO2006040551A2 · 2006 [cited by applicant]
WO WO2004065617A3 · 2006 [cited by applicant]
WO WO2006078841A1 · 2006 [cited by applicant]
WO WO2006096571A2 · 2006 [cited by applicant]
WO WO2007081385A2 · 2007 [cited by applicant]
WO WO2007081387A1 · 2007 [cited by applicant]
WO WO2007089541A2 · 2007 [cited by applicant]
WO WO2007133710A2 · 2007 [cited by applicant]
WO WO2007140015A2 · 2007 [cited by applicant]
WO WO2007147079A2 · 2007 [cited by applicant]
WO WO2008021123A1 · 2008 [cited by applicant]
WO WO2007147079A3 · 2008 [cited by applicant]
WO WO2008109176A2 · 2008 [cited by applicant]
WO WO2008121342A2 · 2008 [cited by applicant]
WO WO2008134153A1 · 2008 [cited by applicant]
WO WO2008150432A1 · 2008 [cited by applicant]
WO WO2009011808A1 · 2009 [cited by applicant]
WO WO2009015296A1 · 2009 [cited by applicant]
WO WO2009085215A1 · 2009 [cited by applicant]
WO WO2009152928A2 · 2009 [cited by applicant]
WO WO2010033200A2 · 2010 [cited by applicant]
WO WO2010104604A1 · 2010 [cited by applicant]
WO WO2010117620A2 · 2010 [cited by applicant]
WO WO2010148039A2 · 2010 [cited by applicant]
WO WO2011028539A1 · 2011 [cited by applicant]
WO WO2011047870A1 · 2011 [cited by applicant]
WO WO2011056546A1 · 2011 [cited by applicant]
WO WO2011066476A1 · 2011 [cited by applicant]
WO WO2010148039A3 · 2011 [cited by applicant]
WO WO2012048341A1 · 2012 [cited by applicant]
WO WO2012061832A1 · 2012 [cited by applicant]
WO WO2012083225A2 · 2012 [cited by applicant]
WO WO2012083225A3 · 2012 [cited by applicant]
WO WO2012106546A2 · 2012 [cited by applicant]
WO WO2012112804A1 · 2012 [cited by applicant]
WO WO2012112970A2 · 2012 [cited by applicant]
WO WO2012116331A2 · 2012 [cited by applicant]
WO WO2012142531A2 · 2012 [cited by applicant]
WO WO2012142611A2 · 2012 [cited by applicant]
WO WO2012149042A2 · 2012 [cited by applicant]
WO WO2012166425A2 · 2012 [cited by applicant]
WO WO2012167142A2 · 2012 [cited by applicant]
WO WO2013019751A1 · 2013 [cited by applicant]
WO WO2012116331A3 · 2013 [cited by applicant]
WO WO2013036929A1 · 2013 [cited by applicant]
WO WO2013055955A1 · 2013 [cited by applicant]
WO WO2013096643A1 · 2013 [cited by applicant]
WO WO2013126741A1 · 2013 [cited by applicant]
WO WO2013134261A1 · 2013 [cited by applicant]
WO WO2012106546A3 · 2013 [cited by applicant]
WO WO2014028378A2 · 2014 [cited by applicant]
WO WO2012142531A3 · 2014 [cited by applicant]
WO WO2012166425A3 · 2014 [cited by applicant]
WO WO2014108810A2 · 2014 [cited by applicant]
WO WO2014165559A2 · 2014 [cited by applicant]
WO WO2014108810A3 · 2014 [cited by applicant]
WO WO2015015199A2 · 2015 [cited by applicant]
WO WO2015044428A1 · 2015 [cited by applicant]
WO WO2015095226A2 · 2015 [cited by applicant]
WO WO2015164212A1 · 2015 [cited by applicant]
WO WO2012142611A3 · 2016 [cited by applicant]
WO WO2016040476A1 · 2016 [cited by applicant]
WO WO2016061517A2 · 2016 [cited by applicant]
WO WO2016061517A3 · 2016 [cited by applicant]
WO WO2016126871A2 · 2016 [cited by applicant]
WO WO2016168584A1 · 2016 [cited by applicant]
WO WO2017015075A1 · 2017 [cited by applicant]
WO WO2017066231A1 · 2017 [cited by applicant]
WO WO2017171985A1 · 2017 [cited by applicant]
WO WO2017180949A1 · 2017 [cited by applicant]
WO WO2017184707A1 · 2017 [cited by applicant]
WO WO2017197343A2 · 2017 [cited by applicant]
WO WO2018039338A1 · 2018 [cited by applicant]
WO WO2018064640A1 · 2018 [cited by applicant]
WO WO2018091676A1 · 2018 [cited by applicant]
WO WO2018119301A1 · 2018 [cited by applicant]
WO WO2018119447A2 · 2018 [cited by applicant]
WO WO2018172726A1 · 2018 [cited by applicant]
WO WO2018191701A1 · 2018 [cited by applicant]
WO WO2018213643A1 · 2018 [cited by applicant]
WO WO2018226546A1 · 2018 [cited by applicant]
WO WO2018236615A1 · 2018 [cited by applicant]
WO WO2019028166A1 · 2019 [cited by applicant]
WO WO2019040637A1 · 2019 [cited by applicant]
WO WO2019083852A1 · 2019 [cited by applicant]
WO WO2019084043A1 · 2019 [cited by applicant]
WO WO2019084165A1 · 2019 [cited by applicant]
WO WO2019108851A1 · 2019 [cited by applicant]
WO WO2019113235A1 · 2019 [cited by applicant]
WO WO2019118355A1 · 2019 [cited by applicant]
WO WO2019126789A1 · 2019 [cited by applicant]
WO WO2019148042A1 · 2019 [cited by applicant]
WO WO2019152108A1 · 2019 [cited by applicant]
WO WO2019157529A1 · 2019 [cited by applicant]
WO WO2019165318A1 · 2019 [cited by applicant]
WO WO2019169028A1 · 2019 [cited by applicant]
WO WO2019169347A1 · 2019 [cited by applicant]
WO WO2019191321A1 · 2019 [cited by applicant]
WO WO2019217758A1 · 2019 [cited by applicant]
WO WO2020028882A1 · 2020 [cited by applicant]
WO WO2020041148A1 · 2020 [cited by applicant]
WO WO2020142779A1 · 2020 [cited by applicant]
WO WO2020168013A1 · 2020 [cited by applicant]
WO WO2020198532A1 · 2020 [cited by applicant]
WO WO2021046475A1 · 2021 [cited by applicant]
WO WO2021133845A1 · 2021 [cited by applicant]
Cited By (18)
US 12,275,993 US 12,305,239 US 12,312,640 US 12,365,893 US 12,391,975 US 12,391,976 US 12,398,262 US 12,416,102 US 12,467,088 US 12,480,158 US 12,534,760 US 12,553,074 US 12,600,961 US 12,606,860 US 12,612,714 US 12,655,450 US 12,686,949 US 12,692,536